公开(公告)号：EP2203567A1

公开(公告)日：2010-07-07

申请号：EP08839669.2

申请日：2008-10-16

申请人： Roche Diagnostics GmbH ,  F. Hoffmann-La Roche AG

发明人： HIGUCHI, Russell, Gene ,  ERLICH, Henry A. ,  BENTLEY, Gordon

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6881 ,  C12Q1/6855 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2563/179 ,  C12Q2525/149 ,  C12Q2565/537

摘要： The invention provides methods and reagent for performing full, multi-locus HLA genotyping for multiple individuals in a single sequencing run using clonal sequencing.

公开(公告)号：EP2480683B1

公开(公告)日：2017-11-29

申请号：EP10757165.5

申请日：2010-09-17

申请人： Roche Diagnostics GmbH ,  F. Hoffmann-La Roche AG

发明人： BENTLEY, Gordon ,  ERLICH, Henry A. ,  GOODRIDGE, Damian ,  LADNER, Martha ,  TRACHTENBERG, Elizabeth

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q1/6881 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2600/172 ,  G06F19/00 ,  Y02A90/26

摘要： Disclosed is a method of determining KIR genotypes for one or more individuals in parallel, the method comprising: for each individual, amplifying the polymorphic exon sequences of the KIR genes, pooling the KIR amplicons, performing emulsion PCR followed by pyrosequencing in parallel to determine all the amplicon sequences present in the individual to determine which KIR alleles are present in the individual.

公开(公告)号：EP2203567B1

公开(公告)日：2011-05-11

申请号：EP08839669.2

申请日：2008-10-16

申请人： Roche Diagnostics GmbH ,  F. Hoffmann-La Roche AG

发明人： HIGUCHI, Russell, Gene ,  ERLICH, Henry A. ,  BENTLEY, Gordon

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6881 ,  C12Q1/6855 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2563/179 ,  C12Q2525/149 ,  C12Q2565/537

摘要： The invention provides methods and reagent for performing full, multi-locus HLA genotyping for multiple individuals in a single sequencing run using clonal sequencing.

公开(公告)号：EP2419530A1

公开(公告)日：2012-02-22

申请号：EP10714201.0

申请日：2010-04-15

申请人： Roche Diagnostics GmbH ,  F.Hoffmann-La Roche AG

发明人： BENTLEY, Gordon ,  ERLICH, Henry, A. ,  HIGUCHI, Russell, Gene ,  HOLCOMB, Cherie

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6881 ,  C12Q1/6869 ,  C12Q2600/156 ,  C12Q2535/122

摘要： A method for detecting one or more HLA sequence types is described that comprises the steps of: amplifying a plurality of first amplicons from a double stranded nucleic acid sample, wherein the first amplicons are amplified with a plurality of pairs of nucleic acid primers that define exons 2 and 3 of both strands of HLA loci from the group consisting of HLA-A, HLA-B, and HLA-C; amplifying the first amplicons to produce a plurality of populations of second amplicons, wherein each population of second amplicons is clonally amplified from one of the first amplicons; sequencing the plurality of populations of second amplicons to generate a nucleic acid sequence composition for each of the plurality of second amplicons; and detecting variation in the sequence composition from one or more of the second amplicons for one or more of the HLA loci..

公开(公告)号：EP2480683A1

公开(公告)日：2012-08-01

申请号：EP10757165.5

申请日：2010-09-17

申请人： Roche Diagnostics GmbH ,  F.Hoffmann-La Roche AG

发明人： BENTLEY, Gordon ,  ERLICH, Henry A. ,  GOODRIDGE, Damian ,  LADNER, Martha ,  TRACHTENBERG, Elizabeth

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q1/6881 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2600/172 ,  G06F19/00 ,  Y02A90/26

摘要： Disclosed is a method of determining KIR genotypes for one or more individuals in parallel, the method comprising: for each individual, amplifying the polymorphic exon sequences of the KIR genes, pooling the KIR amplicons, performing emulsion PCR followed by pyrosequencing in parallel to determine all the amplicon sequences present in the individual to determine which KIR alleles are present in the individual.

摘要翻译： 公开了一种平行测定一个或多个个体的K​​IR基因型的方法，所述方法包括：对于每个个体，扩增KIR基因的多态性外显子序列，汇集KIR扩增子，进行乳液PCR，然后并行进行焦磷酸测序以确定所有 存在于个体中的扩增子序列以确定个体中存在哪些KIR等位基因。