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1.发明授权PREVENTING ENDOGENOUS AMINOPEPTIDASE MEDIATED N-TERMINAL AMINO ACID CLEAVAGE DURING EXPRESSION OF FOREIGN GENES IN BACTERIA 失效预防N-末端氨基BY内源氨肽酶分裂外源基因在细菌中的mRNA表达
公开(公告)号：EP0670907B1
公开(公告)日：1997-05-07
申请号：EP94901914.5
申请日：1993-11-29
发明人： HEITZMANN, Markus , MOVVA, Rao , RAMAGE, Paul
CPC分类号： C12N15/10 , C07K14/54 , C07K14/5412 , C12P21/06
摘要： A process for the recombinant preparation in a bacterial host of the mature form of a mammalian protein or peptide of formula X-Pro-Z, which is subject to processing by endogenous bacterial aminopeptidases is provided. In the formula X-Pro-Z, X is a single N-terminal amino acid other than proline, and Z is the remaining sequence of amino acid residues of the protein or peptide. The process comprises inserting into a cell of the bacterial host an appropriate vector containing DNA coding for Met-Y-X-Pro-Z, in which Y is a natural amino acid that is specifically cleavable in vitro from X-Pro-Z by an aminopeptidase and that imparts resistance to in vivo processing by endogenous bacterial aminopeptidases. The cell is then induced to express the expression product Met-Y-X-Pro-Z which is then treated with an appropriate aminopeptidase to cleave off Met and Y.
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2.发明公开PREVENTING ENDOGENOUS AMINOPEPTIDASE MEDIATED N-TERMINAL AMINO ACID CLEAVAGE DURING EXPRESSION OF FOREIGN GENES IN BACTERIA 失效预防N-末端氨基BY内源氨肽酶分裂外源基因在细菌中的mRNA表达。
公开(公告)号：EP0670907A1
公开(公告)日：1995-09-13
申请号：EP94901914.0
申请日：1993-11-29
发明人： HEITZMANN, Markus , MOVVA, Rao , RAMAGE, Paul
CPC分类号： C12N15/10 , C07K14/54 , C07K14/5412 , C12P21/06
摘要： A process for the recombinant preparation in a bacterial host of the mature form of a mammalian protein or peptide of formula X-Pro-Z, which is subject to processing by endogenous bacterial aminopeptidases is provided. In the formula X-Pro-Z, X is a single N-terminal amino acid other than proline, and Z is the remaining sequence of amino acid residues of the protein or peptide. The process comprises inserting into a cell of the bacterial host an appropriate vector containing DNA coding for Met-Y-X-Pro-Z, in which Y is a natural amino acid that is specifically cleavable in vitro from X-Pro-Z by an aminopeptidase and that imparts resistance to in vivo processing by endogenous bacterial aminopeptidases. The cell is then induced to express the expression product Met-Y-X-Pro-Z which is then treated with an appropriate aminopeptidase to cleave off Met and Y.
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