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1.发明公开LIQUID MEDIUM FOR NERVE CELLS, PROCESS FOR PRODUCING THE SAME AND METHOD FOR INCUBATING NERVE CELLS WITH THE USE OF THE SAME 失效液体介质神经细胞，其制造方法相同，孵化神经细胞使用该方法
公开(公告)号：EP0776968A1
公开(公告)日：1997-06-04
申请号：EP96921088.9
申请日：1996-06-26
IPC分类号： C12N5/06
CPC分类号： C12N5/0619 , C12N2500/25 , C12N2500/84 , C12N2500/90 , C12N2501/392 , C12N2502/08
摘要： The present invention is directed to a culture medium for neurons prepared by adding albumin to a culture supernatant obtained from a culture of primary astroglial cells in a trophic medium supplemented with insulin and transferrin.
The culture medium of the present invention makes it possible to culture central nerve cells consistently. When they are cultured at a low cell density, neurites extend excellently, and synapses are formed rapidly. On the other hand, when they are cultured at a high cell density, long-term stability of cells that had formed neuronetworks can be obtained.摘要翻译： 本发明涉及一种培养基中加入白蛋白到在补充有胰岛素，转一营养培养基从初级星形胶质细胞的培养而获得的培养物上清液制备的神经元。 本发明的培养基中，能够培养中枢神经细胞一致。 当它们是在低细胞密度培养时，神经突极好地延伸，和突触正在迅速形成。 在另一方面，当他们在高密度细胞培养，细胞的长期稳定并已形成神经网络可以得到。
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2.发明授权LIQUID MEDIUM FOR NERVE CELLS, PROCESS FOR PRODUCING THE SAME AND METHOD FOR INCUBATING NERVE CELLS WITH THE USE OF THE SAME 失效液体介质神经细胞，其制造方法相同，孵化神经细胞使用该方法
公开(公告)号：EP0776968B1
公开(公告)日：2004-10-13
申请号：EP96921088.9
申请日：1996-06-26
CPC分类号： C12N5/0619 , C12N2500/25 , C12N2500/84 , C12N2500/90 , C12N2501/392 , C12N2502/08
摘要： A liquid medium for nerve cells prepared by adding albumin to the culture supernatant of primary astroglia cells obtained by incubating the astroglia cells in a nutritional medium containing insulin and transferrin. By using the liquid medium, central nerve cells can be stably incubated. Thus, in low density incubation, synapses excellent in the elongation of axons can be quickly formed, while, in high density incubation, it is possible to provide cells forming a nervous network and being excellent in the stability over a prolonged storage.
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