公开(公告)号：EP3027737B1

公开(公告)日：2018-12-26

申请号：EP14744087.9

申请日：2014-07-25

Applicant: F. Hoffmann-La Roche AG

Inventor： BAILLY, Jacques ,  CIAMPI, Osele ,  GRAF, Martin ,  IACONE, Roberto ,  PATSCH, Christoph

IPC: C12N5/071

CPC classification number: C12N5/0687 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2501/155 ,  C12N2501/385 ,  C12N2501/392 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2506/45

Abstract: This application relates to a method for differentiating pluripotent stem cells (PSCs) into multi-competent renal precursor cells expressing Six2. These renal precursor cells are able to differentiate into fully functional and fully differentiated podocytes. Moreover this application relates to a method for differentiating human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) into defined renal precursor cells expressing Six2 and podocytes based on linked steps of chemically defined medium inductions.

公开(公告)号：EP2898065B1

公开(公告)日：2018-11-14

申请号：EP13766954.5

申请日：2013-09-23

Applicant: Plasticell Limited

Inventor： TARUNINA, Marina ,  CHOO, Yen ,  MYLVAGANAM, Jeyakumar ,  WATSON, Thomas ,  ROSEELL, Meritxell ,  HOOK, Lilian ,  HERNANDEZ, Diana

IPC: C12N5/077 ,  A61K35/35

CPC classification number: C12N5/0653 ,  A61K35/35 ,  C12N2500/24 ,  C12N2500/25 ,  C12N2501/01 ,  C12N2501/04 ,  C12N2501/155 ,  C12N2501/33 ,  C12N2502/1352 ,  C12N2506/1384

Abstract: The present invention relates to brown adipose tissue (BAT) cells derived from adult stern or progenitor cells, derived from adult white fat tissue (WAT), as well as to methods for deriving such cells.

公开(公告)号：EP3347451A1

公开(公告)日：2018-07-18

申请号：EP16778499.0

申请日：2016-09-09

Applicant: The General Hospital Corporation

Inventor： REN, Xi ,  OTT, Harald C.

IPC: C12N5/071 ,  C12M3/00

CPC classification number: C12N5/0697 ,  C12M21/08 ,  C12M25/14 ,  C12M41/48 ,  C12N5/0688 ,  C12N2500/02 ,  C12N2500/25 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/17 ,  C12N2501/39 ,  C12N2501/415 ,  C12N2502/1388 ,  C12N2502/27 ,  C12N2502/28 ,  C12N2506/45 ,  C12N2533/92

Abstract: A method for vascular regeneration comprises delivering endothelial cells to a lung scaffold, delivering perivascular cells to the lung scaffold, and providing a multiphase culture program to the scaffold. The multiphase culture program comprises a first phase including delivering an angiogenic medium, e.g., having 40-100 ng/ml of pro-angiogenic factors, and a second phase including delivering a stabilization medium, e.g., having 0.5-2% of serum and 1-20 ng/ml of angiogenic factors.

公开(公告)号：EP2931875B1

公开(公告)日：2018-03-21

申请号：EP13812432.6

申请日：2013-12-12

Applicant: DePuy Synthes Products, Inc.

Inventor： BHATIA, Ravinder ,  HONG, L.S. Klaudyne ,  OZTURK, Sadettin S. ,  KAMARAJU, Venkat H.

IPC: C12N5/00 ,  C12N5/073

CPC classification number: C12N5/0605 ,  C12N5/0037 ,  C12N5/0075 ,  C12N5/0665 ,  C12N2500/25 ,  C12N2500/33 ,  C12N2500/38

Abstract: This invention provides for methods of growing anchorage-dependent cells (e.g. hUTC) in culture medium comprising amino acids, vitamins, salts nucleosides, insulin, transferrin, ethanolamine and sodium selenium, wherein the culture medium is supplemented with serum. The method further comprises addition of a serum-free nutrient solution comprising amino acids, vitamins, salts nucleosides, insulin, transferrin, ethanolamine and sodium selenium. The invention also provides for culture media and serum-free nutrient solutions for growing anchorage-dependent cells.

公开(公告)号：EP2185691B1

公开(公告)日：2018-03-14

申请号：EP08837420.2

申请日：2008-07-31

Applicant: LifeScan, Inc.

Inventor： O'NEIL, John, J.

IPC: C12N5/071

CPC classification number: C12N5/0676 ,  C12N2500/02 ,  C12N2500/22 ,  C12N2500/25 ,  C12N2500/44 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2502/22 ,  C12N2506/02

Abstract: The present invention relates to the field of pluripotent stem cell differentiation. The present invention provides methods for the differentiation of pluripotent stem cells on a human feeder cell layer. In particular, the present invention provides an improved method for the differentiation of pluripotent stem cells into pancreatic endocrine cells using a human feeder cell layer.

公开(公告)号：EP2029725B1

公开(公告)日：2018-01-03

申请号：EP07797729.6

申请日：2007-05-24

Applicant: The University of North Carolina at Chapel Hill

Inventor： KUBOTA, Hiroshi ,  REID, Lola, M.

IPC: C12N5/074

CPC classification number: C12N5/0018 ,  C12N5/067 ,  C12N5/0672 ,  C12N2500/00 ,  C12N2500/05 ,  C12N2500/20 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/00 ,  C12N2501/11 ,  C12N2501/235 ,  C12N2501/33 ,  G01N33/56966

Abstract: The present invention relates to precursor cells to hepatic stellate cells, compositions comprising same and methods of isolating same. The surface antigenic profile of the precursors is MHC class Ia negative, ICAM-1 + , VCAM-1 + , ²3-integrin + . In addition to expression of these surface markers, the cells also express the intracellular markers desmin, vimentin, smooth muscle ±-actin, nestin, hepatocyte growth factor, stromal derived factor-1± and Hlx homeobox transcriptional factor.

公开(公告)号：EP3260140A1

公开(公告)日：2017-12-27

申请号：EP17170810.0

申请日：2012-12-05

Applicant: Factor Bioscience Inc.

Inventor： ANGEL, Matthew ,  ROHDE, Christopher

IPC: A61K48/00 ,  C07H21/02 ,  C12N15/00

CPC classification number: C12N5/0647 ,  A61K35/28 ,  A61K2035/124 ,  C08G77/08 ,  C08K5/5399 ,  C12N5/0657 ,  C12N9/16 ,  C12N9/22 ,  C12N15/87 ,  C12N15/907 ,  C12N2500/25 ,  C12N2500/44 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/2303 ,  C12N2501/26 ,  C12N2501/91 ,  C12N2501/998 ,  C12N2506/09 ,  C12N2800/80 ,  C12P21/00 ,  C12Y301/21 ,  H01L31/048 ,  Y02E10/50 ,  C08L83/04

Abstract: The present invention relates in part to nucleic acids encoding proteins, nucleic acids containing non-canonical nucleotides, therapeutics comprising nucleic acids, methods, kits, and devices for inducing cells to express proteins, methods, kits, and devices for transfecting, gene editing, and reprogramming cells, and cells, organisms, and therapeutics produced using these methods, kits, and devices. Methods for inducing cells to express proteins and for reprogramming and gene-editing cells using RNA are disclosed. Methods for producing cells from patient samples, cells produced using these methods, and therapeutics comprising cells produced using these methods are also disclosed.

Abstract translation: 本发明部分涉及编码蛋白质的核酸，含有非规范核苷酸的核酸，包含核酸的治疗剂，诱导细胞表达蛋白质的方法，试剂盒和装置，方法，试剂盒和用于转染，基因编辑， 并重编程细胞，以及使用这些方法，试剂盒和设备产生的细胞，生物体和治疗剂。 公开了诱导细胞表达蛋白质和使用RNA进行重编程和基因编辑细胞的方法。 还公开了从患者样品产生细胞的方法，使用这些方法产生的细胞以及包含使用这些方法产生的细胞的治疗剂。

公开(公告)号：EP2456854B1

公开(公告)日：2017-08-30

申请号：EP10726999.5

申请日：2010-07-02

Applicant: Oncobiotek

Inventor： ROUYER, Nicolas, Jacques ,  BARTHEL, Robert

IPC: C12N5/077

CPC classification number: C12N5/0656 ,  C12N2500/25 ,  C12N2500/90 ,  C12N2501/11

公开(公告)号：EP3198005A1

公开(公告)日：2017-08-02

申请号：EP15777723.6

申请日：2015-09-24

Applicant: The University Court of the University of Edinburgh

Inventor： TELFER, Evelyn Elizabeth ,  MCLAUGHLIN, Marie

IPC: C12N5/075 ,  C12N5/071

CPC classification number: C12N5/0611 ,  A01N1/0268 ,  C12N5/0018 ,  C12N5/0037 ,  C12N5/0081 ,  C12N5/0609 ,  C12N5/0682 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/125 ,  C12N2501/16 ,  C12N2501/31 ,  C12N2501/392 ,  C12N2502/243 ,  C12N2527/00

Abstract: Methods of preparing ovarian tissue for primordial follicle growth are presented comprising the steps: providing an ovarian tissue sample comprising cortical tissue and stromal tissue; removing damaged tissue from the ovarian tissue sample where present; removing excess stromal tissue from the ovarian tissue sample where present; and then mechanically stretching the ovarian tissue sample along at least one dimension of the ovarian tissue sample, such that the size of the ovarian tissue sample along the at least one dimension is increased by at least 10%. Methods of growing viable oocyte in vitro, and methods of preparing individual ovarian follicles for growth are also presented.

Abstract translation: 提出了制备用于原始卵泡生长的卵巢组织的方法，包括以下步骤：提供包含皮质组织和基质组织的卵巢组织样品; 从存在的卵巢组织样本中去除受损组织; 在存在的情况下从卵巢组织样品中除去过量的基质组织; 然后沿卵巢组织样本的至少一个维度机械拉伸卵巢组织样本，使得卵巢组织样本沿着至少一个维度的大小增加至少10％。 还介绍了体外培养活卵母细胞的方法，以及制备用于生长的单个卵巢卵泡的方法。

公开(公告)号：EP3152300A1

公开(公告)日：2017-04-12

申请号：EP15732068.0

申请日：2015-06-05

Applicant: Pluriomics B.v.

Inventor： BRAAM, Stefan Robbert ,  TEROOLEN, Leon ,  MUMMERY, Christine

IPC: C12N5/077

CPC classification number: C12N5/0657 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/35 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2500/99 ,  C12N2501/01 ,  C12N2501/125 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/165 ,  C12N2501/30 ,  C12N2501/395 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2533/90

Abstract: This disclosure generally concerns the fields of cell biology and molecular biology. In particular the invention concerns the field of stem cell biology and maturation of stem cell-derived cardiomyocytes. Disclosed is a method for improving the maturity of stem-cell derived cardiomyocytes, in particular of the ventricular type, as can be witnessed by, for example, an improved upstroke velocity of the stem-cell derived cardiomyocytes.

Abstract translation: 本公开一般涉及细胞生物学和分子生物学领域。 特别地，本发明涉及干细胞生物学和干细胞衍生的心肌细胞的成熟领域。 公开了一种用于改善干细胞来源的心肌细胞，尤其是心室型心肌细胞成熟度的方法，正如可以通过例如干细胞来源的心肌细胞的上行速度改善来证明的。