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1.发明公开CROWDING AGENT-INDUCED NUCLEIC ACID TRANSFER INTO A RECIPIENT HOST CELL 审中-公开拥挤，药物引起的NUCLEIC转移到受体宿主细胞
公开(公告)号：EP2890787A4
公开(公告)日：2016-06-29
申请号：EP13833688
申请日：2013-08-30
IPC分类号： C12N15/04
摘要： The presently disclosed invention relates to methods of transferring large nucleic acid molecules or a genome from one cell (the donor) into heterologous host cells in the presence of a crowding agent. The method allows for greater ease and efficiency of transfer of genetic material. Introduction of the donor genetic material into the recipient host cells also allows for manipulation of the donor nucleic acid molecule or genome within the host cells. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells.
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