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    METHODS FOR ENHANCING A SECRETION EFFICIENCY OF RECOMBINANT FOREIGN PROTEIN IN YEAST EXPRESSION SYSTEM
    6.
    发明公开
    METHODS FOR ENHANCING A SECRETION EFFICIENCY OF RECOMBINANT FOREIGN PROTEIN IN YEAST EXPRESSION SYSTEM 有权
    提高酵母表达系统中重组外源蛋白分泌效率的方法

    公开(公告)号：EP2041280A1

    公开(公告)日：2009-04-01

    申请号：EP06768960.4

    申请日：2006-06-20

    申请人： Mogam Biotechnology Research Institute

    发明人： LIM, Hyung-Kwon ,  KIM, Sung-Geun

    IPC分类号： C12N15/10 ,  C12N15/04 ,  C12N9/38

    CPC分类号： C12N15/81

    摘要： Provided is a method for improving secretion efficiency of a recombinant foreign protein in a yeast expression system. The method comprises transforming a yeast host with a recombinant foreign gene construct comprising a galactose-inducible promoter, a secretion signal sequence and a gene encoding the foreign protein to construct a transformed yeast strain; and culturing the transformed yeast strain under the condition that the activity of the galactose-inducible promoter is controlled. Improved secretion efficiency of the foreign protein can be achieved by decreasing over-expression-induced insoluble precipitation of the recombinant foreign protein suffered by a conventional galactose-inducible promoter-based yeast expression system, via appropriate control of a level of galactose functioning as an inducer of the galactose-inducible promoter in cells. Due to improved secretion efficiency of the recombinant foreign protein, present invention makes a contribution to improvement in productivity of recombinant foreign proteins in the yeast expression system and reduction in production costs.

    摘要翻译： 提供了一种改善酵母表达系统中重组外源蛋白分泌效率的方法。 该方法包括用包含半乳糖诱导型启动子，分泌信号序列和编码外源蛋白的基因的重组外源基因构建体转化酵母宿主以构建转化的酵母菌株; 并且在半乳糖诱导型启动子的活性受到控制的条件下培养转化的酵母菌株。 通过适当控制作为诱导剂起作用的半乳糖水平，通过降低常规基于半乳糖诱导型启动子的酵母表达系统所遭受的重组外来蛋白的过表达诱导的不溶性沉淀，可以实现外源蛋白分泌效率的提高 的细胞中半乳糖诱导型启动子。 由于重组外来蛋白质的分泌效率提高，本发明有助于提高酵母表达系统中重组外来蛋白质的生产力并降低生产成本。

    MONOTERPENE SYNTHASES FROM GRAND FIR ($i(ABIES GRANDIS))
    9.
    发明公开
    MONOTERPENE SYNTHASES FROM GRAND FIR ($i(ABIES GRANDIS)) 失效
    FIR的单萜合酶（冷杉桉）

    公开(公告)号：EP1032257A4

    公开(公告)日：2005-03-16

    申请号：EP98935641

    申请日：1998-07-10

    申请人： UNIV WASHINGTON

    发明人： BOHLMANN JOERG ,  STEELE CHRISTOPHER L ,  CROTEAU RODNEY B

    IPC分类号： A23D9/00 ,  C11B1/00 ,  C12N9/88 ,  C12N15/82 ,  A01H5/00 ,  A01H5/10 ,  A23D7/00 ,  A23K1/14 ,  C07H21/04 ,  C07K4/10 ,  C12N15/04 ,  C12N15/63

    CPC分类号： A23D9/00 ,  C11B1/00 ,  C12N9/88 ,  C12N15/8242 ,  C12N15/8243

    摘要： cDNAs encoding myrcene synthase, (-)-limonene synthase and (-)-pinene synthase from Grand fir (Abies grandis) haven been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID NO:1; SEQ ID NO:3 and SEQ ID NO:5) are provided which code for the expression of myrcene synthase (SEQ ID NO:2), (-)-pinene synthase (SEQ ID NO:4) and (-)-limonene synthase (SEQ ID NO:6) , respectively, from Grand fir (Abies grandis). In other aspects, replicable recombinant cloning vehicles are provided which code for myrcene synthase, (-)-limonene synthase and (-)-pinene synthase, or for a base sequence sufficiently complementary to at least a portion of myrcene synthase, (-)-limonene synthase or (-)-pinene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding myrcene synthase, (-)-limonene synthase or (-)-pinene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant monoterpene synthases that may be used to facilitate their production, isolation and purification in significant amounts. Recombinant myrcene synthase, (-)-limonene synthase or (-)-pinene synthase may be used to obtain expression or enhanced expression of myrcene synthase, (-)-limonene synthase or (-)-pinene synthase in plants in order to enhance the production of monoterpenoids, or may be otherwise employed for the regulation or expression of myrcene synthase, (-)-limonene synthase and (-)-pinene synthase, or the production of their products.