公开(公告)号：EP3011062A2

公开(公告)日：2016-04-27

申请号：EP14813675.7

申请日：2014-06-19

申请人： Sample6 Technologies Inc.

发明人： KOERIS, Michael Sandor ,  THOMASON, Julie ,  CAPPILLINO, Michael ,  SHIVERS, Robert Patrick ,  BROWNELL, Daniel Robert ,  BOWERS, Jayson L. ,  LU, Timothy Kuan Ta ,  KRZYMANSKA-OLEJNIK, Edyta

IPC分类号： C12Q1/70

CPC分类号： C12Q1/66 ,  C12N7/00 ,  C12Q1/10 ,  G01N33/82 ,  G01N2333/245 ,  G01N2333/255

摘要： Methods of detecting target bacteria are provided. In some embodiments the methods comprise exposing the sample to a phage capable of infecting a set of target bacteria and comprising a heterologous nucleic acid sequence encoding a marker. In some embodiments the target bacteria comprise Listeria. In some embodiments the target bacteria are all Listeria. Recombinant Listeria phage comprising a heterologous nucleic acid sequence encoding a marker are also provided as are useful combinations of such phage and articles of manufacture comprising such phage, among other things.

公开(公告)号：EP3320108A1

公开(公告)日：2018-05-16

申请号：EP16824938.1

申请日：2016-07-08

申请人： Sample6 Technologies Inc.

发明人： KOERIS, Michael Sandor ,  CAPPILLINO, Michael ,  KRZYMANSKA-OLEJNIK, Edyta ,  BROWNELL, Daniel Robert ,  SHIVERS, Robert Patrick ,  BOWERS, Jayson L. ,  LU, Timothy Kuan-Ta

IPC分类号： C12Q1/04 ,  C12Q1/66

CPC分类号： C12Q1/04 ,  C12N7/00 ,  C12N15/86 ,  C12N2795/00022 ,  C12N2795/00043

摘要： Composition and methods for the detection of one or more target microbe(s) are provided. Compositions of the disclosure include at least one recombinant phage capable of infecting a target microbe, said phage comprising at least a capsid protein sequence, a ribosome binding site, and a codon-optimized marker. Compositions of the disclosure may further include an aqueous solution that enhances the ability to detect marker expression upon phage infection of the target microbe. In some embodiments the target microbe is Listeria.