公开(公告)号：EP1709185A1

公开(公告)日：2006-10-11

申请号：EP04808620.1

申请日：2004-12-29

申请人： Samyang Genex Corporation

发明人： KIM, Chang-Heon ,  SON, Joo-Sun ,  KIM, Jin-Ah ,  YUN, Jeong-Hwan ,  NA, Gwang-Hwee ,  SONG, Jai-Young ,  CHOI, Ho-Joon

IPC分类号： C12P17/02

CPC分类号： C12P15/00

摘要： The present invention relates to a method for mass production of secondary metabolites in plant cell culture, more specifically, to a method for production of secondary metabolites comprising the step of culturing plant cells in a medium treated with alkanoic acid or salt thereof. The present invention can contribute to the commercial production of useful secondary metabolites by providing a method for mass production of secondary metabolites from plant cells.

公开(公告)号：EP0966540B1

公开(公告)日：2003-11-19

申请号：EP98929893.0

申请日：1998-06-25

申请人： Samyang Genex Corporation

发明人： CHOI, Hyung-Kyoon ,  CHOI, Ho-Joon ,  KIM, Sang-Ic ,  YUN, Jeong-Hwan ,  SON, Joo-Sun ,  CHANG, Moon-Seok ,  CHOI, Eunsoo ,  KIM, Hong-Rak ,  HONG, Seung-Suh ,  LEE, Hyun-Soo

IPC分类号： C12P17/02

CPC分类号： C12N5/04 ,  C12P17/02

摘要： The present invention relate to a method to increase the production level of paclitaxel by changing the temperature during the plant cell culture. According to the present invention, the production of the paclitaxel comprises the following procedure: (i) cultivating the Taxus genus plant cells in a medium at ca. 20 to 25 DEG C; and (ii) when growth of the plant cells has progressed sufficiently, changing the cultivation temperature to ca. 26 to 32 DEG C to continue the culture. The present invention comprises also the method of increasing the paclitaxel production by inoculating the cells at a high initial concentration and by increasign the saccharide concentration in the medium. According to the present invention, paclitaxel can be mass-produced conveniently and therefore has an industrial application.