公开(公告)号：EP2720074A2

公开(公告)日：2014-04-16

申请号：EP13188497.5

申请日：2013-10-14

申请人： Spectral Applied Research Inc.

发明人： Berman, Richard

IPC分类号： G02B21/00

CPC分类号： G02B21/0076 ,  G02B21/0032 ,  G02B21/0044

摘要： In an episcopic multiplexed confocal scanning microscope system (400, 450, 500, 600, 700, 800, 900, 1000), a spatial filter (432, 514, 632) is placed at a conjugate plane (448) to a back aperture plane (446) at a back aperture of an objective lens (412). The spatial filter is used to combine excitation light and emission light from a multiplexed confocal microscope module (406).

摘要翻译： 在落射复共聚焦扫描显微镜系统（400，450，500，600，700，800，900，1000），一个空间滤波器（432，514，632）是在一个共轭面（448）到背面开口面放置 （446）在物镜（412）的背面开口。 空间滤波器用于激发光和发射光从一个复用的共焦显微镜模块（406）相结合。

公开(公告)号：EP2720075A1

公开(公告)日：2014-04-16

申请号：EP13188498.3

申请日：2013-10-14

申请人： Spectral Applied Research Inc.

发明人： Berman, Richard ,  Sinclair, Peter Maclean ,  Oreopoulos, John

IPC分类号： G02B21/16 ,  G01N21/64 ,  G02B27/56

CPC分类号： G02B21/0076 ,  G01N21/6458 ,  G01N21/648 ,  G02B21/16 ,  G02B27/56

摘要： A multiple wavelength total internal reflection fluoresence 'TIRF' microscopy system has an objective (304). A dispersion unit (350,650) of the system comprises a high-dispersion optical element (354,654). The dispersion unit receives illumination light having at least a first wavelength λ 1 and a second wavelength λ 2 , where λ 1 ≠ λ 2 , and splits the illumination light into a first monochromatic beam having the first wavelength λ 1 and a second monochromatic beam having the second wavelength λ 2 . The monochromatic beams are focused onto a back focal plane (306) of the objective, near an outer edge of the objective, at different radial distances from an optical axis (346) of the objective. The dispersion unit is rotatable in order to adjust angles of incidence of the monochromatic beams onto an interface (316) between a substrate (312) and a sample (314) to be imaged, wherein the angles of incidence are greater than the critical angle of the interface.

摘要翻译： 多波长全内反射荧光“TIRF”显微镜系统具有目的（304）。 系统的色散单元（350,650）包括高色散光学元件（354,654）。 分散单元接收具有至少第一波长»1和第二波长λ2的照明光，其中»1‰»2，并将照明光分解成具有第一波长»1的第一单色光束和具有第一波长λ的第二单色光束， 第二波长»2。 单色光束在与物镜的光轴（346）不同的径向距离处聚焦到物镜的后焦平面（306）上，靠近物镜的外边缘。 分散单元是可旋转的，以便将单色光束的入射角度调整到待成像的衬底（312）和样品（314）之间的界面（316）上，其中入射角大于临界角 界面。

公开(公告)号：EP2720075B1

公开(公告)日：2017-11-29

申请号：EP13188498.3

申请日：2013-10-14

申请人： Spectral Applied Research Inc.

发明人： Berman, Richard ,  Sinclair, Peter Maclean ,  Oreopoulos, John

IPC分类号： G02B21/16 ,  G01N21/64 ,  G02B27/56 ,  G02B21/00

CPC分类号： G02B21/0076 ,  G01N21/6458 ,  G01N21/648 ,  G02B21/16 ,  G02B27/56

摘要： A multiple wavelength total internal reflection fluoresence 'TIRF' microscopy system has an objective (304). A dispersion unit (350,650) of the system comprises a high-dispersion optical element (354,654). The dispersion unit receives illumination light having at least a first wavelength » 1 and a second wavelength » 2 , where » 1 ‰ » 2 , and splits the illumination light into a first monochromatic beam having the first wavelength » 1 and a second monochromatic beam having the second wavelength » 2 . The monochromatic beams are focused onto a back focal plane (306) of the objective, near an outer edge of the objective, at different radial distances from an optical axis (346) of the objective. The dispersion unit is rotatable in order to adjust angles of incidence of the monochromatic beams onto an interface (316) between a substrate (312) and a sample (314) to be imaged, wherein the angles of incidence are greater than the critical angle of the interface.

公开(公告)号：EP2720074A3

公开(公告)日：2014-06-04

申请号：EP13188497.5

申请日：2013-10-14

申请人： Spectral Applied Research Inc.

发明人： Berman, Richard

IPC分类号： G02B21/00

CPC分类号： G02B21/0076 ,  G02B21/0032 ,  G02B21/0044

摘要： In an episcopic multiplexed confocal scanning microscope system (400, 450, 500, 600, 700, 800, 900, 1000), a spatial filter (432, 514, 632) is placed at a conjugate plane (448) to a back aperture plane (446) at a back aperture of an objective lens (412). The spatial filter is used to combine excitation light and emission light from a multiplexed confocal microscope module (406).

公开(公告)号：EP2196839B1

公开(公告)日：2014-05-14

申请号：EP09405217.2

申请日：2009-12-08

申请人： Spectral Applied Research Inc.

发明人： Berman, Richard

IPC分类号： G02B21/00

CPC分类号： G02B21/0032 ,  G02B6/06 ,  G02B21/00 ,  G02B21/002 ,  G02B21/0024 ,  G02B21/0036

公开(公告)号：EP2196839A8

公开(公告)日：2011-12-28

申请号：EP09405217.2

申请日：2009-12-08

申请人： Spectral Applied Research Inc.

发明人： Berman, Richard

IPC分类号： G02B21/00

CPC分类号： G02B21/0032 ,  G02B6/06 ,  G02B21/00 ,  G02B21/002 ,  G02B21/0024 ,  G02B21/0036

摘要： A multi-mode optical fiber (1126) delivers light from a radiation source to a multi-focal confocal microscope (1102) with reasonable efficiency. A core diameter of the multi-mode fiber is selected such that an etendue of light emitted from the fiber is not substantially greater than a total etendue of light passing through a plurality of pinholes in a pinhole array of the multi-focal confocal microscope. The core diameter may be selected taking into account a specific optical geometry of the multi-focal confocal microscope, including pinhole diameter and focal lengths of relevant optical elements. For coherent radiation sources, phase randomization may be included. A multi-mode fiber enables the use of a variety of radiation sources and wavelengths in a multi-focal confocal microscope, since the coupling of the radiation source to the multi-mode fiber is less sensitive to mechanical and temperature influences than coupling the radiation source to a single mode fiber.