公开(公告)号：EP3256254A1

公开(公告)日：2017-12-20

申请号：EP16714564.8

申请日：2016-02-04

申请人： Stichting VU

发明人： BIEBRICHER, Andreas Sebastian ,  CANDELLI, Andrea ,  HELLER, Iddo ,  LAURENS, Niels ,  PETERMAN, Erwin Johannes Gerard ,  WUITE, Gijs Jan Lodewijk

IPC分类号： B01L3/00 ,  G02B21/32

CPC分类号： G02B21/32 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0654 ,  B01L2400/0454 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6439 ,  G02B21/16 ,  G02B21/18 ,  G02B21/361 ,  G02B26/0816 ,  G02B27/106 ,  G02B27/141 ,  G02B27/283

摘要： The present disclosure relates to an apparatus (100) and method for controlling a plurality of simultaneously active optical traps (OT1, OT2, OT3). In one method, trapping beams (TB1, TB2, TB3) are provided and redirected for individually controlling a respective position (X,Y) of optical traps (OT1, OT2, OT3) formed by focusing of the redirected trapping beams in a sample volume (SV). Light (L11,L20) from the sample volume (SV) corresponding to the optical traps is received. A path of a detector beam (AB) is overlapped with one of the trapping beams (TB3), wherein the detector beam has a distinct wavelength (λA) from that of the overlapping trapping beam (TB3). In one channel, the light from the sample volume is filtered according to wavelength, and only the filtered light having the wavelength (λA) of the detector beam (AB) is measured.

公开(公告)号：EP3256254B1

公开(公告)日：2018-12-12

申请号：EP16714564.8

申请日：2016-02-04

申请人： Stichting VU

发明人： BIEBRICHER, Andreas Sebastian ,  CANDELLI, Andrea ,  HELLER, Iddo ,  LAURENS, Niels ,  PETERMAN, Erwin Johannes Gerard ,  WUITE, Gijs Jan Lodewijk

IPC分类号： G02B21/32 ,  G01B21/18 ,  G02B21/16 ,  G02B27/10 ,  B01L3/00 ,  G02B21/36 ,  G02B27/14 ,  G02B27/28

CPC分类号： G02B21/32 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0654 ,  B01L2400/0454 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6439 ,  G02B21/16 ,  G02B21/18 ,  G02B21/361 ,  G02B26/0816 ,  G02B27/106 ,  G02B27/141 ,  G02B27/283

摘要： The present disclosure relates to an apparatus (100) and method for controlling a plurality of simultaneously active optical traps (OT1,OT2,OT3). In one method, trapping beams (TB1,TB2,TB3) are provided and redirected for individually controlling a respective position (X,Y) of optical traps (OT1,OT2,OT3) formed by focusing of the redirected trapping beams in a sample volume (SV). Light (L11,L20) from the sample volume (SV) corresponding to the optical traps is received. A path of a detector beam (AB) is overlapped with one of the trapping beams (TB3), wherein the detector beam has a distinct wavelength (λA) from that of the overlapping trapping beam (TB3). In one channel, the light from the sample volume is filtered according to wavelength, and only the filtered light having the wavelength (λA) of the detector beam (AB) is measured.

公开(公告)号：EP3004848A1

公开(公告)日：2016-04-13

申请号：EP14731835.6

申请日：2014-06-02

申请人： Stichting VU-VUmc

发明人： WUITE, Gijs Jan Lodewijk ,  PETERMAN, Erwin Johannes Gerard ,  HELLER, Iddo ,  SITTERS, Gerrit ,  CANDELLI, Andrea ,  HELL, Stefan Walter

IPC分类号： G01N21/64 ,  G02B21/00 ,  G02B21/16 ,  G02B21/32 ,  B01L3/00 ,  C12Q1/68

CPC分类号： G01N21/6428 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0877 ,  B01L2400/0454 ,  C12Q1/6816 ,  G01N21/6458 ,  G02B21/0048 ,  G02B21/0076 ,  G02B21/16 ,  G02B21/32 ,  C12Q2565/629

摘要： The present disclosure concerns a method and system for imaging a molecular strand (MS). The method comprises providing a sample volume (SV) comprising the strand (MS); providing an excitation beam (EB) having an excitation focus (EF) in the sample volume (SV); scanning the excitation focus (EF) in the sample volume (SV) along a one dimensional scanning line (SL); trapping an end of the strand (MS) in the sample volume (SV) and extending the strand (MS) along a one-dimensional trapping line (LL) parallel to the scanning line (SL); aligning the trapping line (LL) to coincide with the scanning line (SL) to have the scanning excitation focus (EF) coincide with the strand (MS); and recording the fluorescence response (FR) as a function of a plurality of distinct scanning positions (X0) of the excitation focus (EF) along the scanning line (SL).

摘要翻译： 本公开涉及用于对分子链（MS）进行成像的方法和系统。 该方法包括提供包含链（MS）的样品体积（SV）; 提供在样本体积（SV）中具有激发焦点（EF）的激发光束（EB）; 沿着一维扫描线（SL）扫描样品体积（SV）中的激发聚焦（EF）; 将样品体积（SV）中的股线（MS）的末端捕获并沿着平行于扫描线（SL）的一维捕获线（LL）延伸股线（MS）; 对准捕获线（LL）与扫描线（SL）重合以使扫描激发聚焦（EF）与链（MS）重合; 以及沿着扫描线（SL）记录作为激发焦点（EF）的多个不同扫描位置（X0）的函数的荧光响应（FR）。