公开(公告)号：EP1422509A1

公开(公告)日：2004-05-26

申请号：EP03256930.3

申请日：2003-10-31

申请人： Streck Laboratories, Inc.

发明人： Ryan, Wayne L. ,  Wu, Jiong

IPC分类号： G01N1/30 ,  G01N33/50 ,  G01N33/72

CPC分类号： G01N33/5094 ,  C12N1/06 ,  G01N1/30 ,  Y10T436/107497

摘要： A reagent system for substantially lysing red blood cells in a whole blood sample prior to leukocyte analysis, the reagent system includes: a first reagent for substantially lysing the red blood cells in the whole blood sample, and a second reagent for quenching the activity of the first reagent, wherein the second reagent includes a base and has a pH value of about 8 to 12. A final acidic media, ranging from about pH 4 to about 6, is used to stabilize the white blood cells and continuously remove red blood cell fragments. The first reagent is formulated to include: a saponin compound; an acid selected from the group consisting of halogenated carboxylic acids, phosphoric acid or combinations thereof and optionally a surfactant. In addition a method of lysing the red blood cells present in a sample of whole blood, includes the steps of: combining a predetermined portion of the sample of whole blood with a predetermined portion of a first reagent for substantially lysing the red blood cells and stabilizing white blood cells in the whole blood sample, wherein the first reagent includes: a saponin compound; and an acid; and quenching the lysing action of said first reagent by the addition of a predetermined portion of a second reagent, wherein the second reagent includes a base and has a pH value of about 8 to about 12 to give a solution containing substantially lysed red blood cells, leukocytes and a pH value of about 3 to about 6.

摘要翻译： 一种试剂系统，用于在白细胞分析之前基本上裂解全血样品中的红细胞，所述试剂系统包括：用于基本上裂解全血样品中的红细胞的第一试剂和用于猝灭全血样品的活性的第二试剂 第一试剂，其中第二试剂包括碱并且具有约8至12的pH值。使用约pH 4至约6的最终酸性介质来稳定白细胞并连续除去红细胞碎片 。 第一试剂配制成包括：皂苷化合物; 选自卤代羧酸，磷酸或其组合和任选的表面活性剂的酸。 此外，溶解全血样品中的红细胞的方法包括以下步骤：将全血样品的预定部分与第一试剂的预定部分组合，以基本上裂解红细胞并稳定 全血样品中的白细胞，其中第一试剂包括：皂苷化合物; 和酸; 并通过加入第二试剂的预定部分淬灭所述第一试剂的裂解作用，其中第二试剂包括碱，pH值为约8至约12，得到含有基本上裂解的红细胞的溶液， 白细胞和约3至约6的pH值