公开(公告)号：EP1816461B1

公开(公告)日：2020-01-08

申请号：EP07008752.3

申请日：2003-10-16

申请人： Streck Laboratories, Inc.

发明人： Ryan, Wayne L.

IPC分类号： G01N1/31 ,  A01N1/00 ,  B01L3/14

公开(公告)号：EP1816461A1

公开(公告)日：2007-08-08

申请号：EP07008752.3

申请日：2003-10-16

申请人： Streck Laboratories, Inc.

发明人： Ryan, Wayne L.

IPC分类号： G01N1/31 ,  A01N1/00 ,  B01L3/14

摘要： The claimed subject matter comprises a device to collect and preserve cells comprising of: (a) a collection container (10) comprised of a tube (12) having an open end (14) and a closed end (16), a closure (18) in the open end of the tube, a vacuum drawn to a predetermined level inside the container; and, (b) compounds (22) including an anticoagulant agent (24) and a fixative agent (22), wherein the compounds (22) are in a sufficient amount to preserve said cells' original morphology and antigenic sites without significant dilution of said cells, thereby allowing said cells to be directly analyzed by a flow cytometer without further treatment. More particularly the ratio of said compounds (22) over a final composition (30) comprising said cells (20) and said compounds (22) is less than about 2:100 (volume/volume). The claimed subject matter further includes a method of producing said contained (10) and a method for preparing cells for analysis comprising in which said cells are collected in said container (10).

摘要翻译： 要求保护的主题包括装置收集和保存的细胞，其包括：（a）组成的管（12），具有一开口端（14）和封闭端（16），一封闭件的收集容器（10）（18 ）在管，吸引到容器内的预定水平的真空的开口端; 和（b）的化合物（22）包含在抗凝血剂（24）和worin化合物固定剂（22）（22）是在一个足够的量以维持所述细胞的原始形态和抗原性位点没有所述的显著稀释 细胞，从而允许所述细胞通过流式细胞仪不经进一步处理直接分析。 更特别地，所述的比例，化合物（22）在包含所述细胞（20）的最终组合物（30）和所述化合物（22）小于约2：100（体积/体积）。 要求保护的主题还包括含有产生所述的方法（10）和用于制备细胞以进行分析的方法，包括，其中所述细胞在所述容器（10）被收集。

公开(公告)号：EP1422509A1

公开(公告)日：2004-05-26

申请号：EP03256930.3

申请日：2003-10-31

申请人： Streck Laboratories, Inc.

发明人： Ryan, Wayne L. ,  Wu, Jiong

IPC分类号： G01N1/30 ,  G01N33/50 ,  G01N33/72

CPC分类号： G01N33/5094 ,  C12N1/06 ,  G01N1/30 ,  Y10T436/107497

摘要： A reagent system for substantially lysing red blood cells in a whole blood sample prior to leukocyte analysis, the reagent system includes: a first reagent for substantially lysing the red blood cells in the whole blood sample, and a second reagent for quenching the activity of the first reagent, wherein the second reagent includes a base and has a pH value of about 8 to 12. A final acidic media, ranging from about pH 4 to about 6, is used to stabilize the white blood cells and continuously remove red blood cell fragments. The first reagent is formulated to include: a saponin compound; an acid selected from the group consisting of halogenated carboxylic acids, phosphoric acid or combinations thereof and optionally a surfactant. In addition a method of lysing the red blood cells present in a sample of whole blood, includes the steps of: combining a predetermined portion of the sample of whole blood with a predetermined portion of a first reagent for substantially lysing the red blood cells and stabilizing white blood cells in the whole blood sample, wherein the first reagent includes: a saponin compound; and an acid; and quenching the lysing action of said first reagent by the addition of a predetermined portion of a second reagent, wherein the second reagent includes a base and has a pH value of about 8 to about 12 to give a solution containing substantially lysed red blood cells, leukocytes and a pH value of about 3 to about 6.

摘要翻译： 一种试剂系统，用于在白细胞分析之前基本上裂解全血样品中的红细胞，所述试剂系统包括：用于基本上裂解全血样品中的红细胞的第一试剂和用于猝灭全血样品的活性的第二试剂 第一试剂，其中第二试剂包括碱并且具有约8至12的pH值。使用约pH 4至约6的最终酸性介质来稳定白细胞并连续除去红细胞碎片 。 第一试剂配制成包括：皂苷化合物; 选自卤代羧酸，磷酸或其组合和任选的表面活性剂的酸。 此外，溶解全血样品中的红细胞的方法包括以下步骤：将全血样品的预定部分与第一试剂的预定部分组合，以基本上裂解红细胞并稳定 全血样品中的白细胞，其中第一试剂包括：皂苷化合物; 和酸; 并通过加入第二试剂的预定部分淬灭所述第一试剂的裂解作用，其中第二试剂包括碱，pH值为约8至约12，得到含有基本上裂解的红细胞的溶液， 白细胞和约3至约6的pH值

公开(公告)号：EP1413874A1

公开(公告)日：2004-04-28

申请号：EP03256535.0

申请日：2003-10-16

申请人： Streck Laboratories, Inc.

发明人： Ryan, Wayne L.

IPC分类号： G01N1/31 ,  A01N1/00 ,  B01L3/14

CPC分类号： A01N1/0226 ,  B01L3/5082 ,  B01L9/54 ,  B01L2200/16

摘要： The claimed subject matter comprises a device to collect and preserve cells comprising of: (1) a collection container comprised of a tube having an open end and a closed end, a closure in the open end of the tube, a vacuum drawn to a predetermined level inside the container; and (2) compounds including an anticoagulant agent and a fixative agent, wherein the compounds are in a sufficient amount to preserve said cells' original morphology and antigenic sites without significant dilution of said cells, and thereby allowing said cells to be directly analyzed by a flow cytometer without further treatment. The claimed subject matter further comprises of a method of making a collection device for cells comprising of : (1) providing a tube having an open end and a closed end; (2) preloading compounds including: an anticoagulant agent, and a fixative agent into the tube, wherein the compounds are in a sufficient amount to preserve the cells' original morphology and antigenic sites without significant dilution of the cells, and thereby allowing the cells to be directly analyzed by a flow cytometer without further treatment; (3) inserting a closure into the open end of the tube; and (4) drawing a vacuum inside the tube to a predetermined level to form the collection device.

摘要翻译： 通过提供管具有开口端和封闭端，预加载包含抗凝血剂和固定剂进入管中，其中所述化合物保留细胞的原始形态和抗原性位点没有细胞的显著稀释化合物，插入封盖进入的开口端使得收集装置用于细胞 管; 和抽真空内管以形成所述收集装置。 对于细胞制备收集装置（100）包括提供管（12）具有开口端（14）和封闭端（16），预压包括在抗凝血剂的化合物，和固定剂（I）插入管，所述 从重氮烷基脲，咪唑烷基脲，dimethoylol -5,5-二甲基乙选择固定剂，二羟甲基脲，2-溴-2-硝基丙烷-1,3-二醇，恶唑烷酮，钠羟甲基甘氨酸盐，5- hydroxymethoxymethyl-1-1aza-3， 7-二氧杂二环[3.3.0]辛烷，5-羟甲基-3,7-二氧杂二环1-1aza- [3.3.0]辛烷-5-羟基聚[亚甲基氧基]甲基-3,7-二氧杂二环1-1aza- [3.3.0 ]辛烷，季金刚烷和它们的组合，其中所述化合物是在一个足够的量以保持细胞的原始形态和抗原位点，而不细胞显著稀释，并由此使细胞通过流动直接分析仪而无需进一步处理 ，插入一个封闭件（18）插入所述管的开口端，并抽真空桶的内部 e的预定电平，以形成所述收集装置。 因此独立权利要求中包括了以下内容：（1）收集的细胞，其包括收集容器（10），包括具有开口端和封闭端，在管的开口端的封闭件，抽成真空的管装置 在容器内的预定电平，并包括对抗凝剂和（I）在管内的化合物; 和（2）一种试剂盒，其包括所述设备，并且还包括酒精拭子，纱布，管保持器，止血带，手套，其他细胞收集管中，针，刺血针，粘合带，注射器，测试条，条含试剂用于细胞分析，包装单元 存储用于至少一个部件和所述收集装置以形成试剂盒，或用于输送收集装置的包装单元。

公开(公告)号：EP1217372A1

公开(公告)日：2002-06-26

申请号：EP01204186.9

申请日：2001-11-02

申请人： Streck Laboratories, Inc.

发明人： Ryan, Wayne L. ,  Turpen, Paula B.

IPC分类号： G01N33/50 ,  G01N1/30

CPC分类号： G01N1/30 ,  G01N33/5094 ,  Y10T436/105831 ,  Y10T436/107497 ,  Y10T436/108331

摘要： An improved reagent system for preparation of cells for flow cytometry having a physiologically compatible salt solution composition including a lysing agent, an agent for minimizing white blood cell lysis, and optionally a preservative.

摘要翻译： 用于制备流式细胞术的细胞的改进的试剂系统具有生理上相容的盐溶液组合物，所述组合物包含裂解剂，用于最小化白细胞裂解的试剂和任选的防腐剂。