公开(公告)号：EP4458954A1

公开(公告)日：2024-11-06

申请号：EP22916047.8

申请日：2022-12-26

申请人： Sumitomo Pharma Co., Ltd.

发明人： TAHARA, Masahiro ,  FUJIKI, Ayaka ,  YOSHIDA, Kenji ,  KITAGAWA, Maiko

IPC分类号： C12N5/079 ,  A61K35/28 ,  A61P43/00 ,  C07K14/78 ,  C12N5/0775 ,  C12Q1/6851 ,  C12N5/10 ,  C12N15/12

摘要： The present invention aims to provide a culturing method and a method for producing a cell population containing a neural crest cell, the methods being able to stably mass produce the neural crest cell from a pluripotent stem cell with quality that allows the cell to be used in a therapy with a cell, and a method for producing a mesenchymal stem cell and the like using the neural crest cell. The method for selectively culturing a neural crest cell in a cell population containing a neural crest cell and/or a neural crest progenitor cell of the present invention comprises a culturing step of adherent culturing the cell population containing the neural crest cell and/or the neural crest progenitor cell, in the presence of one or more extracellular matrices selected from the group consisting of laminin with its α chain being an α1 chain and its β chain being a β1 chain, laminin with its α chain being an α2 chain and its β chain being a β1 chain, laminin with its α chain being an α2 chain and its β chain being a β2 chain, and laminin with its α chain being an α5 chain and its β chain being a β1 chain, and extracellular matrices containing integrin-binding sites of these laminins.