专利检索 ap:("Swedish Orphan Biovitrum AB (Publ)") AND inv:"STRÖMQUIST, Thomas" 第 1 页

1.

发明公开
LOW PH PROTEIN PURIFICATION PROCESS 审中-公开
标题翻译：方法在蛋白质具有低pH清洗

公开(公告)号：EP2710126A1

公开(公告)日：2014-03-26

申请号：EP12785836.3

申请日：2012-05-15

申请人： Swedish Orphan Biovitrum AB (Publ)

发明人： STRÖMQUIST, Thomas , WOOD, Susanne

IPC分类号： C12N9/18 , B01D15/32 , B01D15/36 , C07K1/18 , C07K1/20

CPC分类号： C12N9/20 , B01D15/327 , B01D15/363

摘要： The invention relates to a process for recovering and purifying bile salt-stimulated lipase (BSSL) in a solution which contains impurities, said process comprising the steps: (i) applying BSSL to a hydrophobic interaction chromatography (HIC) resin; (ii)removing impurities by washing said HIC resin with a wash composition having a pH in the range from 4 to 5; and (iii) recovering BSSL from said HIC resin.