公开(公告)号：EP3268457A1

公开(公告)日：2018-01-17

申请号：EP16762649.8

申请日：2016-03-11

申请人： Synthetic Genomics, Inc.

发明人： CAIAZZA, Nicky, C. ,  FELNAGLE, Elizabeth, A. ,  URANO, Jun ,  WIN, Maung, N. ,  RADAKOVITS, Randor, R.

IPC分类号： C11B1/00 ,  C12N1/00 ,  C12N1/15 ,  C12P7/64

CPC分类号： C12N9/93 ,  A23D9/00 ,  A23K10/12 ,  A23K20/158 ,  C12N9/0071 ,  C12P7/6427 ,  C12Y114/19 ,  C12Y602/01003

摘要： Recombinant microorganisms engineered for the production of polyunsaturated fatty acids (PUFAs) are provided. Also provided are biomass, microbial oils, and food products and ingredients produced by or comprising the microorganisms of the invention.

公开(公告)号：EP3265567A1

公开(公告)日：2018-01-10

申请号：EP16759329.2

申请日：2016-02-29

申请人： Synthetic Genomics, Inc.

发明人： CAIAZZA, Nicky, C. ,  WIN, Maung, Nyan ,  URANO, Jun

IPC分类号： C12N15/63 ,  C12N15/80 ,  C12N15/113 ,  C12Q1/68

摘要： The present disclosure generally relates to novel polynucleotide molecules for use in regulating gene expression in recombinant cells, such as labyrinthulomycetes cells. The disclosure further relates to nucleic acid constructs, such as vectors and expression cassettes, containing a regulatory element operably linked to a heterologous nucleotide sequence. The disclosure further relates to methods for stably transforming a host cell, such as a labyrinthulomycetes cell with transgenes. Stably transformed recombinant cells, progeny, biomaterials derived therefrom, and methods for preparing and using the same are also provided.

公开(公告)号：EP3535385A1

公开(公告)日：2019-09-11

申请号：EP17868420.5

申请日：2017-10-31

申请人： Synthetic Genomics, Inc.

发明人： CAIAZZA, Nicky, C.

IPC分类号： C12N1/19 ,  C12N15/09 ,  C12N15/80 ,  C12P21/00

公开(公告)号：EP2931913B1

公开(公告)日：2018-10-17

申请号：EP13863073.6

申请日：2013-12-11

申请人： Synthetic Genomics, Inc.

发明人： URANO, Jun ,  GIBSON, Daniel, G. ,  CAIAZZA, Nicky, C. ,  QI, Zhiqing

IPC分类号： C12N15/10 ,  C12P19/34 ,  C12Q1/6844

CPC分类号： C12P19/34 ,  C12N15/1027 ,  C12N15/1031 ,  C12Q1/6844 ,  C12Q2521/101 ,  C12Q2527/101 ,  C12Q2527/125 ,  C12Q2527/143 ,  C12Q2533/107

摘要： The present invention discloses methods for assembling a nucleic acid molecule from a set of overlapping oligonucleotides. The method involves contacting a set of overlapping oligonucleotides with a DNA polymerase, a mixture of dNTPs, and a crowding agent to form an assembly mixture. In one embodiment the crowding agent is polyethylene glycol (PEG). The presence of the crowding agent facilitates the nucleic acid assembly process of the invention. The assembly mixture is then subjected to multiple cycles, each cycle comprising an annealing phase, an extension phase, and a denaturation phase, and the desired nucleic acid molecule is thereby assembled. In some embodiments one or more of the phases are time varied.

公开(公告)号：EP2931913A1

公开(公告)日：2015-10-21

申请号：EP13863073.6

申请日：2013-12-11

申请人： Synthetic Genomics, Inc.

发明人： URANO, Jun ,  GIBSON, Daniel, G. ,  CAIAZZA, Nicky, C. ,  QI, Zhiqing

IPC分类号： C12P19/34 ,  C12Q1/68 ,  C12N15/00

CPC分类号： C12P19/34 ,  C12N15/1027 ,  C12N15/1031 ,  C12Q1/6844 ,  C12Q2521/101 ,  C12Q2527/101 ,  C12Q2527/125 ,  C12Q2527/143 ,  C12Q2533/107

摘要： The present invention discloses methods for assembling a nucleic acid molecule from a set of overlapping oligonucleotides. The method involves contacting a set of overlapping oligonucleotides with a DNA polymerase, a mixture of dNTPs, and a crowding agent to form an assembly mixture. In one embodiment the crowding agent is polyethylene glycol (PEG). The presence of the crowding agent facilitates the nucleic acid assembly process of the invention. The assembly mixture is then subjected to multiple cycles, each cycle comprising an annealing phase, an extension phase, and a denaturation phase, and the desired nucleic acid molecule is thereby assembled. In some embodiments one or more of the phases are time varied.