公开(公告)号：EP1167524B1

公开(公告)日：2007-05-16

申请号：EP00908056.5

申请日：2000-03-14

申请人： TAKARA BIO INC.

发明人： MUKAI, Hiroyuki ,  YAMAMOTO, Junko ,  TAKEDA, Osamu ,  MIYAKE, Kazue ,  UEMORI, Takashi ,  SATO, Yoshimi ,  MORIYAMA, Mariko ,  SAWARAGI, Haruhisa ,  HAGIYA, Michio ,  ASADA, Kiyozo ,  KATO, Ikunoshin

IPC分类号： C12N15/11 ,  C12Q1/68

CPC分类号： C12Q1/6844 ,  C12Q2531/119 ,  C12Q2525/121 ,  C12Q2521/319

摘要： A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.