METHOD OF AMPLIFYING NUCLEIC ACID
    1.
    发明公开
    METHOD OF AMPLIFYING NUCLEIC ACID 有权
    VERFAHREN ZURNUKLEINSÄUREAMPLIFIKATION

    公开(公告)号:EP1715037A1

    公开(公告)日:2006-10-25

    申请号:EP04820173.5

    申请日:2004-12-06

    申请人: TAKARA BIO INC.

    IPC分类号: C12N15/00 C12Q1/68

    摘要: In the case where a template nucleic acid has sequences substantially the same as each other, a primer is designed in a region between these sequences so as to give a product in a ladder structure in which a target region is polymerized in a single sequence via the same sequences in the ICAN reaction. By using a chimeric oligonucleotide primer and a ladder-forming oligonucleotide primer containing a specific base sequence, an amplification product having a ladder structure can be positively formed and thus the sensitivity, amplification efficiency and reaction speed in the ICAN reaction can be elevated.

    摘要翻译: 在模板核酸具有彼此基本相同的序列的情况下,在这些序列之间的区域中设计引物,以便产生梯形结构中的产物,其中靶区域通过经由 在ICAN反应中相同的序列。 通过使用嵌合寡核苷酸引物和含有特定碱基序列的梯形寡核苷酸引物,可以积极地形成具有梯形结构的扩增产物,从而提高ICAN反应中的灵敏度,扩增效率和反应速度。

    METHOD OF ANALYZING GENE INTRODUCTION SITE
    2.
    发明公开
    METHOD OF ANALYZING GENE INTRODUCTION SITE 审中-公开
    VERFAHREN ZUR ANALYZE EINERGENEINFÜRUNGSSTELLE

    公开(公告)号:EP1621638A1

    公开(公告)日:2006-02-01

    申请号:EP04731764.9

    申请日:2004-05-07

    申请人: TAKARA BIO INC.

    IPC分类号: C12Q1/68

    CPC分类号: C12Q1/6844 C12Q2527/107

    摘要: A method of analyzing, in chromosomal DNA having a retroviral vector incorporated therein, DNA region derived from chromosome which is adjacent to the vector, characterized in that a primer extension reaction and a nucleic acid amplification reaction are carried out in the presence of a compound capable of lowering the Tm value of double stranded nucleic acid. There are further provided a kit and buffer for use in this method.

    摘要翻译: 一种在其中掺入了逆转录病毒载体的染色体DNA中分析衍生自与载体相邻的染色体的DNA区域的方法,其特征在于引物延伸反应和核酸扩增反应是在有化合物存在的条件下进行的 降低双链核酸的Tm值。 还提供了一种用于该方法的试剂盒和缓冲液。