公开(公告)号：EP1762609B1

公开(公告)日：2012-04-04

申请号：EP06019209.3

申请日：2006-09-13

申请人： TAKARA BIO INC.

发明人： Yoshioka, Hirofumi ,  Katayama, Yasushi ,  Tomura, Daisuke ,  Funakoshi, Masao ,  Matsumoto, Kazuya ,  Mineno, Junichi ,  Takesako, Kazutoh ,  Kato, Ikunoshin

IPC分类号： C12N5/00 ,  C12N5/10

CPC分类号： C12N5/0037 ,  C12N2500/84 ,  C12N2510/02

公开(公告)号：EP1762609A3

公开(公告)日：2007-05-02

申请号：EP06019209.3

申请日：2006-09-13

申请人： TAKARA BIO INC.

发明人： Yoshioka, Hirofumi ,  Katayama, Yasushi ,  Tomura, Daisuke ,  Funakoshi, Masao ,  Matsumoto, Kazuya ,  Mineno, Junichi ,  Takesako, Kazutoh ,  Kato, Ikunoshin

IPC分类号： C12N5/06

CPC分类号： C12N5/0037 ,  C12N2500/84 ,  C12N2510/02

摘要： A method for obtaining a virus vector free of a serum, and a serum-free medium for cultivation of a virus producer cell which can be used for the method are provided. By cultivating a virus producer cell using a serum-free medium containing serum albumin, it is possible to cultivate the cell in a state equivalent to that in a serum-containing medium to produce a virus vector at a sufficient titer. The virus vector prepared from the virus producer cell cultivated in the medium exhibits gene transfer efficiency comparable to that of a conventional vector. The medium is useful for gene therapy and studies thereof.

公开(公告)号：EP1762609A2

公开(公告)日：2007-03-14

申请号：EP06019209.3

申请日：2006-09-13

申请人： TAKARA BIO INC.

发明人： Yoshioka, Hirofumi ,  Katayama, Yasushi ,  Tomura, Daisuke ,  Funakoshi, Masao ,  Matsumoto, Kazuya ,  Mineno, Junichi ,  Takesako, Kazutoh ,  Kato, Ikunoshin

IPC分类号： C12N5/06

CPC分类号： C12N5/0037 ,  C12N2500/84 ,  C12N2510/02

摘要： A method for obtaining a virus vector free of a serum, and a serum-free medium for cultivation of a virus producer cell which can be used for the method are provided. By cultivating a virus producer cell using a serum-free medium containing serum albumin, it is possible to cultivate the cell in a state equivalent to that in a serum-containing medium to produce a virus vector at a sufficient titer. The virus vector prepared from the virus producer cell cultivated in the medium exhibits gene transfer efficiency comparable to that of a conventional vector. The medium is useful for gene therapy and studies thereof.

摘要翻译： 本发明提供了获得无血清病毒载体的方法，以及用于培养可用于该方法的病毒生产细胞的无血清培养基。 通过使用含有血清白蛋白的无血清培养基培养病毒生成细胞，可以以与含血清培养基相同的状态培养细胞，以产生足够的滴度的病毒载体。 从培养基中培养的病毒生产细胞制备的病毒载体表现出与常规载体相当的基因转移效率。 该介质可用于基因治疗及其研究。