公开(公告)号：EP0141620A3

公开(公告)日：1988-02-03

申请号：EP84307326

申请日：1984-10-24

申请人： TECHNICON INSTRUMENTS CORPORATION (a New York corporation)

发明人： Siegel, Richard C. ,  Marx, Christina S. ,  Hargitay, Bartholomew ,  Wotherspoon, Neil

IPC分类号： G01N33/543 ,  G01N33/546 ,  G01N33/577

CPC分类号： G01N33/54393 ,  Y10S436/808

摘要： A reagent for a specific binding assay, such as an immunoassay, is prepared by reacting both a partner of a specific binding pair and at least one hydrophilic substance, with haloalkyl moieties associated with the surface of a solid phase, and then recovering the reagent so prepared for any unreacted binding partner. Preferably, the alpha-haloalkyl is chloromethyl and the at least one hydrophilic substance comprises at least one protein, such as aibumin, and at least one non-proteinaceous amine to which the solid phase is impermeable, such as tris (hydroxymethyl) aminomethane. The specific binding assay reagent of the invention can be combined with other reagents to provide an assay composition.