公开(公告)号：EP1221617A2

公开(公告)日：2002-07-10

申请号：EP01650155.3

申请日：2001-12-31

申请人： THE PROVOST, FELLOWS AND SCHOLARS OF THE COLLEGE OF THE HOLY AND UNDIVIDED TRINITY OF QUEEN ELIZABETH NEAR DUBLIN

发明人： Shvets, Igor ,  Kashanin, Dmitriy ,  Kelleher, Dermot ,  Williams, Vivienne ,  Volkov, Yuri

IPC分类号： G01N33/543 ,  G01N33/50 ,  B01L3/00

CPC分类号： G01N33/5005 ,  B01L3/5027 ,  B01L3/502761 ,  B01L3/5635 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/163 ,  B01L2400/0487

摘要： Biological assays using various constructions of biochips are disclosed to mirror in vivo situations. The biochip 50 comprises a microchannel 51 having a liquid outlet port 1, bubble release port 2 and a liquid outlet port 3 with an associated bubble release port 4. A multiplicity of tests can be performed often by coating the bore of the microchannel 50 with various adhesion mediating proteins or the use of chemoattractants. The assay assembly 60 comprises a syringe pump feeding the biochip 50. An inverted microscope 65, digital camera 66 and recorder 67 are provided. A sample liquid containing cells in suspension is injected slowly through the biochip and the effect of the assay recorded over a long period of time.

摘要翻译： 公开了使用生物芯片的各种构造的生物测定法来体现体内情况。 生物芯片50包括具有液体出口1，气泡释放口2和具有相关联的气泡释放口4的液体出口3的微通道51.可以经常通过用各种方式涂覆微通道50的孔来进行多次测试 粘附介导蛋白或使用化学引诱物。 测定组件60包括供给生物芯片50的注射泵。提供倒置显微镜65，数字照相机66和记录器67。 将包含悬浮液中的细胞的样品液体缓慢注入生物芯片，并且长时间记录测定的效果。

公开(公告)号：EP1221617A3

公开(公告)日：2007-08-29

申请号：EP01650155.3

申请日：2001-12-31

申请人： THE PROVOST, FELLOWS AND SCHOLARS OF THE COLLEGE OF THE HOLY AND UNDIVIDED TRINITY OF QUEEN ELIZABETH NEAR DUBLIN

发明人： Shvets, Igor ,  Kashanin, Dmitriy ,  Kelleher, Dermot ,  Williams, Vivienne ,  Volkov, Yuri

IPC分类号： G01N33/543 ,  G01N33/50 ,  B01L3/00

CPC分类号： G01N33/5005 ,  B01L3/5027 ,  B01L3/502761 ,  B01L3/5635 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/163 ,  B01L2400/0487

摘要： Biological assays using various constructions of biochips are disclosed to mirror in vivo situations. The biochip 50 comprises a microchannel 51 having a liquid outlet port 1, bubble release port 2 and a liquid outlet port 3 with an associated bubble release port 4. A multiplicity of tests can be performed often by coating the bore of the microchannel 50 with various adhesion mediating proteins or the use of chemoattractants. The assay assembly 60 comprises a syringe pump feeding the biochip 50. An inverted microscope 65, digital camera 66 and recorder 67 are provided. A sample liquid containing cells in suspension is injected slowly through the biochip and the effect of the assay recorded over a long period of time.