公开(公告)号：EP1932911A1

公开(公告)日：2008-06-18

申请号：EP06797474.1

申请日：2006-09-05

Applicant: THE RESEARCH FOUNDATION FOR MICROBIAL DISEASES OF OSAKA UNIVERSITY

Inventor： TAKEMOTO, Masaya ,  MORI, Yasuko ,  YAMANISHI, Koichi ,  FUKE, Isao ,  GOMI, Yasuyuki ,  TAKAHASHI, Michiaki

IPC: C12N15/09 ,  A01K67/027 ,  A61K39/00 ,  A61P7/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12P21/02

CPC classification number: C12N15/85 ,  A61K2039/53 ,  C12N2710/16522 ,  C12N2830/008 ,  C12N2830/60

Abstract: It is intended to provide a promoter for inducing expression selectively and strongly in an immunocompetent cell and/or a blood cell such as a lymphocyte. In the invention, the object was achieved by finding that HHV6 MIE promoter, HHV7 MIE promoter and HHV7 U95 promoter unexpectedly induce a specific expression in an immunocompetent cell and/or a blood cell such as a T lymphocyte. By utilizing the promoters, a selective delivery of a DNA vaccine or the like can be realized.

Abstract translation: 旨在提供用于在免疫活性细胞和/或血细胞如淋巴细胞中选择性和强烈诱导表达的启动子。 在本发明中，目的是通过发现HHV6MIE启动子，HHV7MIE启动子和HHV7U95启动子在免疫活性细胞和/或血细胞如T淋巴细胞中意外诱导特异性表达来实现。 通过利用启动子，可以实现DNA疫苗的选择性递送等。

公开(公告)号：EP0839911B1

公开(公告)日：2004-07-28

申请号：EP97922068.8

申请日：1997-05-15

Applicant: THE RESEARCH FOUNDATION FOR MICROBIAL DISEASES OF OSAKA UNIVERSITY

Inventor： MORI, Chisato ,  TAKAHARA, Rie ,  OSAME, Juichiro ,  GOMI, Yasuyuki ,  FUKE, Isao

IPC: C12N15/38 ,  C12Q1/70 ,  C12N7/00 ,  C07K14/04 ,  A61K39/25

CPC classification number: C07K14/005 ,  A61K39/00 ,  C12N2710/16722 ,  C12Q1/701

Abstract: A novel and dependable method for identifying an attenuated chickenpox virus Oka strain or a strain originating therein and acceptable as an attenuated chickenpox vaccine virus which comprises distinguishing the above-mentioned Oka strain from a specimen by examining whether the chickenpox virus genomic DNA of the Oka strain agrees with that of the specimen in all of the eight requirements as will be defined hereinbelow or disagrees therewith in at least one of these eight requirements so as to identify the Oka strain: the size of the restriction enzyme Hpa I fragment K; the size of the restriction enzyme Eco RI fragment P; the size determination of the Gene 14 R2-487 region; the PCR-SSCP determination of this region; the size of the restriction enzyme AccIII fragment in the R2-1764 region: the occurrence of the restriction enzyme Pst I site in this region; the homology of the amino acid sequence encoded by the R2-487 region; and the homology of the amino acid sequence encoded by the entire ORF of VZV Gene 14. Also provided are a virus strain substantially identical to the attenuated chickenpox virus Oka strain or a strain originating therein and acceptable as an attenuated chickenpox vaccine virus which has been identified by the above-mentioned method; an attenuated chickenpox vaccine with the use of the virus strain as the active ingredient; and a gp V antigen encoded by Gene 14 which is useful as a diagnostic agent.

公开(公告)号：EP0839911A1

公开(公告)日：1998-05-06

申请号：EP97922068.8

申请日：1997-05-15

Applicant: THE RESEARCH FOUNDATION FOR MICROBIAL DISEASES OF OSAKA UNIVERSITY

Inventor： MORI, Chisato ,  TAKAHARA, Rie ,  OSAME, Juichiro ,  GOMI, Yasuyuki ,  FUKE, Isao

IPC: C12N15/38 ,  C12Q1/70 ,  C12N7/00 ,  C07K14/04 ,  A61K39/25

CPC classification number: C07K14/005 ,  A61K39/00 ,  C12N2710/16722 ,  C12Q1/701

Abstract: Disclosed is a method for exact identification of the attenuated varicella virus Oka strain or a strain derived therefrom capable of functioning as an attenuated varicella live vaccine virus, which comprises analyzing the difference in the genomic DNA and fragments thereof between the Oka strain and a sample varicella strain, and determining whether or not a sample strain satisfies all of the following eight characteristics: the sizes of the Hpa I-K fragment and the Eco RI-P fragment; the size of R2-487 region of Gene14 and the analysis by PCR-SSCP; the sizes of the restriction fragments obtained by digesting the R2-1764 fragment with Acc III; the absence or presence of a Pst I cleavage site; the homology of the amino acid sequences coded by R2-487 coding region; and the homology of the amino acid sequences coded by the coding region of VZV Gene14 . Also disclosed are an isolated virus strain which is substantially the same virus strain as identified by the above method; an attenuated varicella virus live vaccine comprising the same virus strain as identified by the above method; and an attenuated varicella virus Oka strain antigen.

Abstract translation: 公开了一种用于减毒水痘病毒Oka株的准确识别或从能够作为减毒水痘活疫苗病毒，其包含Oka株和样品水痘之间分析所述基因组DNA及其片段的区别它们的作用的衍生有一个应变的方法 应变，和确定性采矿是否样品应变SATIS外资企业所有的以下八个特征：对HPA-K片段和的EcoRI-P片段的大小; Gene14并通过PCR-SSCP分析R2-487的区域的尺寸; 通过用消化的的AccIII片段R2-1764获得的限制性片段的大小; 不存在或一个裂解位点的PstI的存在; 通过R2-487编码区编码的氨基酸序列的同源性; 和氨基酸序列的同源性由VZV Gene14的编码区编码。 所以圆盘游离缺失是在分离的病毒株的所有其基本上作为鉴定通过上述方法相同的病毒株; 的减毒水痘病毒活疫苗，其包含鉴定通过上述方法相同的病毒株; 和减毒水痘病毒Oka株抗原。