公开(公告)号：EP1961814A1

公开(公告)日：2008-08-27

申请号：EP05809552.2

申请日：2005-11-24

Applicant: THE RESEARCH FOUNDATION FOR MICROBIAL DISEASES OF OSAKA UNIVERSITY ,  National Institute of Biomedical Innovation

Inventor： MORI, Yasuko ,  SOMBOONTHUM, Pranee ,  YOSHII, Hironori ,  GOMI, Yasuyuki ,  TAKAHASHI, Michiaki ,  YAMANISHI, Koichi

IPC: C12N15/00

CPC classification number: A61K39/25 ,  A61K39/12 ,  A61K39/165 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/70 ,  C12N15/86 ,  C12N2710/16734 ,  C12N2710/16743 ,  C12N2710/16762 ,  C12N2760/18734 ,  C12N2800/204 ,  C12N2800/50 ,  C12N2820/55 ,  Y02A50/39 ,  Y02A50/394

Abstract: The problems to be solved by the present invention are to provide: a recombinant varicella-zoster virus; a process for producing the same; a pharmacological composition containing a recombinant varicella-zoster virus; a vector containing a BAC vector sequence in the specific gene of a genomic gene of varicella-zoster virus; cells containing such a vector; a fragment capable of homologous recombination with a genome of varicella-zoster virus; a nucleic acid cassette containing the BAC vector sequence; and a multivalent vaccine. The above problems were solved by developing a process for producing a recombinant varicella-zoster virus, wherein the BAC vector sequence is inserted into a specific virus gene.

Abstract translation: 本发明要解决的问题是提供：重组水痘带状疱疹病毒; 其制造方法; 含有重组水痘带状疱疹病毒的药物组合物; 在水痘带状疱疹病毒的基因组基因的特定基因中含有BAC载体序列的载体; 含有这种载体的细胞; 能够与水痘带状疱疹病毒基因组同源重组的片段; 含有BAC载体序列的核酸盒; 和多价疫苗。 通过开发生产重组水痘带状疱疹病毒的方法解决了上述问题，其中将BAC载体序列插入到特定病毒基因中。

公开(公告)号：EP2474611A1

公开(公告)日：2012-07-11

申请号：EP10813526.0

申请日：2010-09-03

Applicant: National Institute of Biomedical Innovation ,  The Research Foundation for Microbial Diseases of Osaka University

Inventor： MATSUURA, Masaaki ,  TAKEMOTO, Masaya ,  KOSHIZUKA, Tetsuo ,  YAMANISHI, Koichi ,  MORI, Yasuko

IPC: C12N15/09 ,  C12N5/10

CPC classification number: C12N15/85 ,  A61K48/0066 ,  C07K14/005 ,  C12N2710/16522 ,  C12N2710/16541 ,  C12N2740/10043 ,  C12N2830/00 ,  C12N2830/008 ,  C12N2830/60

Abstract: Disclosed is an enhancer for a viral promoter such as a promoter that can induce expression selectively and strongly in immunocompetent cells (e.g., lymphocytes) or blood cells. It is found unexpectedly that an intron has the above-mentioned enhancer activity. Thus, it is found that an enhancer for a promoter, which comprises an intron sequence for a major immediate early gene (MIE) of human herpes virus-6 (HHV-6) (particularly HHV-6B) or a fragment of the intron sequence, has a potent promoter activity.

Abstract translation: 公开了用于病毒启动子的增强子，例如可以在免疫活性细胞（例如淋巴细胞）或血细胞中选择性和强烈诱导表达的启动子。 意外地发现内含子具有上述增强子活性。 因此，发现包含人疱疹病毒-6（HHV-6）（特别是HHV-6B）的主要即时早期基因（MIE）的内含子序列或内含子序列的片段的启动子的增强子 ，具有有效的启动子活性。

公开(公告)号：EP1932911A1

公开(公告)日：2008-06-18

申请号：EP06797474.1

申请日：2006-09-05

Applicant: THE RESEARCH FOUNDATION FOR MICROBIAL DISEASES OF OSAKA UNIVERSITY

Inventor： TAKEMOTO, Masaya ,  MORI, Yasuko ,  YAMANISHI, Koichi ,  FUKE, Isao ,  GOMI, Yasuyuki ,  TAKAHASHI, Michiaki

IPC: C12N15/09 ,  A01K67/027 ,  A61K39/00 ,  A61P7/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12P21/02

CPC classification number: C12N15/85 ,  A61K2039/53 ,  C12N2710/16522 ,  C12N2830/008 ,  C12N2830/60

Abstract: It is intended to provide a promoter for inducing expression selectively and strongly in an immunocompetent cell and/or a blood cell such as a lymphocyte. In the invention, the object was achieved by finding that HHV6 MIE promoter, HHV7 MIE promoter and HHV7 U95 promoter unexpectedly induce a specific expression in an immunocompetent cell and/or a blood cell such as a T lymphocyte. By utilizing the promoters, a selective delivery of a DNA vaccine or the like can be realized.

Abstract translation: 旨在提供用于在免疫活性细胞和/或血细胞如淋巴细胞中选择性和强烈诱导表达的启动子。 在本发明中，目的是通过发现HHV6MIE启动子，HHV7MIE启动子和HHV7U95启动子在免疫活性细胞和/或血细胞如T淋巴细胞中意外诱导特异性表达来实现。 通过利用启动子，可以实现DNA疫苗的选择性递送等。

公开(公告)号：EP1721981A1

公开(公告)日：2006-11-15

申请号：EP05719956.4

申请日：2005-03-03

Applicant: THE RESEARCH FOUNDATION FOR MICROBIAL DISEASES OF OSAKA UNIVERSITY

Inventor： NAGAIKE, Kazuhiro ,  MORI, Yasuko ,  GOMI, Yasuyuki ,  TAKAHASHI, Michiaki ,  YAMANISHI, Kouichi

IPC: C12N15/38 ,  A61K39/25 ,  A61P31/22 ,  C12N5/10 ,  C12N7/00

CPC classification number: A61K39/25 ,  A61K39/12 ,  A61K2039/5254 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/16722 ,  C12N2710/16734 ,  C12N2710/16761 ,  C12N2800/50 ,  C12N2820/55

Abstract: A recombinant varicella-zoster virus; a process for producing the same; a pharmacological composition containing recombinant varicella-zoster virus; a vector containing a genomic gene of varicella-zoster virus and BAC vector sequence; cells containing the above vector; a fragment capable of homologous recombination with a genome of varicella-zoster virus; and a nucleic acid cassette containing the BAC vector sequence. For these, there is provided a process for producing recombinant varicella-zoster virus, comprising use of the BAC vector sequence.

Abstract translation: 重组水痘带状疱疹病毒; 其制造方法; 含有重组水痘带状疱疹病毒的药物组合物; 含有水痘带状疱疹病毒和BAC载体序列的基因组基因的载体; 含有上述载体的细胞; 能够与水痘带状疱疹病毒基因组同源重组的片段; 和含有BAC载体序列的核酸盒。 为此，提供了一种生产重组水痘带状疱疹病毒的方法，其包括使用BAC载体序列。