公开(公告)号：EP1310557A1

公开(公告)日：2003-05-14

申请号：EP02080338.3

申请日：1991-09-13

申请人： THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA ,  THE WISTAR INSTITUTE OF ANATOMY AND BIOLOGY ,  COLORADO STATE UNIVERSITY

发明人： Weiner, David ,  Williams, Robert M. ,  Kieber-Emmons, Thomas ,  Williams, Wiliam ,  Greene, Mark I.

IPC分类号： C12N15/12 ,  C12N15/13 ,  C07K16/00 ,  C07K14/705

CPC分类号： C07K16/1063 ,  A61K39/00 ,  C07K14/005 ,  C07K14/7051 ,  C07K14/70514 ,  C07K14/70539 ,  C07K16/10 ,  C12N2720/12022 ,  C12N2740/16122

摘要： The present invention relates to a method for producing peptides which have biological activity comprising: (i) identifying a region of a protein of interest which is homologous to a member of the immunoglobulin superfamily of molecules; (ii) synthesizing a peptide which comprises amino acid sequence found in the region of homology; and (iii) modifying the synthetic peptide such that it assumes a three-dimensional conformation which is biologically active. The invention is based, in part, on the discovery that a cyclic peptide comprising amino acid sequences of a CDR derived from an antibody directed toward a viral receptor molecule can be used to effectively inhibit the binding of virus to its receptor. The invention is also based in part on the discovery that a cyclic peptide corresponding to a portion of human immunodeficiency virus (HIV) gp120 that is homologous to immunoglobulins may be used to generate an anti-HIV immune response.

摘要翻译： 本发明涉及具有生物活性的肽的生产方法，包括：（i）鉴定与分子的免疫球蛋白超家族的成员同源的目的蛋白质的区域; （ii）合成包含在同源性区域中发现的氨基酸序列的肽; 和（iii）修饰合成肽，使其呈现生物活性的三维构象。 本发明部分基于以下发现：包含衍生自针对病毒受体分子的抗体的CDR的氨基酸序列的环肽可用于有效抑制病毒与其受体的结合。 本发明也部分地基于以下发现：对应于与免疫球蛋白同源的人免疫缺陷病毒（HIV）gp120的一部分的环肽可用于产生抗HIV免疫应答。