专利检索 ap:("THE UNITED STATES OF AMERICA represented by the Department of Commerce") AND inv:"DAEMER, Richard, J." 第 1 页

1.

发明公开
HEPATITIS A VIRUS PURIFIED AND TRIPLY CLONED 失效
标题翻译：纯化的A型肝炎病毒，三克隆。

公开(公告)号：EP0196316A1

公开(公告)日：1986-10-08

申请号：EP85904746.0

申请日：1985-09-18

申请人： THE UNITED STATES OF AMERICA represented by the Department of Commerce

发明人： DAEMER, Richard, J. , FEINSTONE, Stephen, M. , GUST, Ian, D. , PURCELL, Robert, H.

IPC分类号： A61K39 , C12N7

CPC分类号： C12N7/00 , A61K39/00 , A61K39/12 , A61K39/29 , A61K2039/5254 , A61K2039/54 , A61K2039/57 , C12N2770/32434 , C12N2770/32451 , C12N2770/32464 , Y02A50/464 , Y10S514/894

摘要： Human hepatitis A virus (HAV) can be purified by a process in which master seed lots of HM-175 strain of hepatitis A virus are prepared from triply cloned virus by terminal dilution at passage level about 20 or preferably at least 20-30. The clones tested induced minimal or no hepatitis although significant antibody response was provoked in inoculated primates. A method of preparing the triply cloned inoculum is also described.