专利检索 ap:("THE UNIVERSITY OF BRITISH COLUMBIA") AND inv:"HARASYM, Troy" 第 1 页

1.

发明授权
HIGH EFFICIENCY ENCAPSULATION OF NUCLEIC ACIDS IN LIPID VESICLES 失效
标题翻译： NUCLEIC到水泡高效包胶

公开(公告)号：EP1027033B1

公开(公告)日：2009-07-22

申请号：EP98921310.3

申请日：1998-05-14

申请人： THE UNIVERSITY OF BRITISH COLUMBIA

发明人： SEMPLE, Sean, C. , KLIMUK, Sandra, K. , HARASYM, Troy , HOPE, Michael, J. , ANSELL, Steven, M. , CULLIS, Pieter , SCHERRER, Peter , DEBEYER, Dan

IPC分类号： A61K9/127 , A61K31/70

CPC分类号： A61K9/1277 , A61K9/1272 , Y10T428/2984

摘要： Methods for the preparation of a lipid-nucleic acid composition are provided. According to the methods, a mixture of lipids containing a protonatable or deprotonatable lipid, for example an amino lipid and a lipid such as a PEG- or polyamide oligomer-modified lipid is combined with a buffered aqueous solution of a charged therapeutic agent, for example polyanionic nucleic acids, to produce particles in which the therapeutic agent is encapsulated in a lipid vesicle. Surface charges on the lipid particles are at least partially neutralized to provide surface-neutralized lipid-encapsulated compositions of the therapeutic agents. The method permits the preparation of compositions with high ratios of therapeutic agent to lipid and with encapsulation efficiencies in excess of 50 %.