公开(公告)号：EP2954055A2

公开(公告)日：2015-12-16

申请号：EP14706395.2

申请日：2014-02-05

申请人： THE UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC. ,  The United States of America, as represented by The Secretary, Department of Health and Human Services ,  Thermo Fisher Scientific Inc.

发明人： KARPILOW, Jon, Michael ,  OBERSTE, Mark, Steven ,  TRIPP, Ralph, A. ,  TOMPKINS, Stephen, M.

IPC分类号： C12N15/113 ,  C12N5/00

CPC分类号： A61K39/13 ,  C12N7/00 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2320/12 ,  C12N2770/32021 ,  C12N2770/32034 ,  C12N2770/32052 ,  C12N2770/32351 ,  C12N2770/32651

摘要： Provided herein are engineered cell lines. In some embodiments, cells of an engineered cell line have altered expression of a gene and/or altered expression of an miRNA, wherein the altered expression results in increased or decreased production of a virus. The virus is a picomavirus, such as a poliovirus or Enterovirus 71. Also provided herein are methods for using the engineered cells to produce virus, and methods for treating a subject having or at risk of having a viral infection.

摘要翻译： 本文提供了工程细胞系。 在一些实施方案中，工程改造的细胞系的细胞具有改变的基因的表达和/或改变的miRNA表达，其中改变的表达导致病毒的产生增加或减少。 病毒是一种皮微病毒病毒，例如脊髓灰质炎病毒或肠病毒71.本文还提供了使用工程细胞产生病毒的方法，以及用于治疗患有或有风险患有病毒感染的受试者的方法。

公开(公告)号：EP2954055B1

公开(公告)日：2018-04-11

申请号：EP14706395.2

申请日：2014-02-05

申请人： University of Georgia Research Foundation, Inc. ,  The United States of America, as represented by The Secretary, Department of Health and Human Services ,  Thermo Fisher Scientific Inc.

发明人： KARPILOW, Jon, Michael ,  OBERSTE, Mark, Steven ,  TRIPP, Ralph, A. ,  TOMPKINS, Stephen, M.

IPC分类号： C12N15/113 ,  C12N5/00

CPC分类号： A61K39/13 ,  C12N7/00 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2320/12 ,  C12N2770/32021 ,  C12N2770/32034 ,  C12N2770/32052 ,  C12N2770/32351 ,  C12N2770/32651

摘要： Provided herein are engineered cell lines. In some embodiments, cells of an engineered cell line have altered expression of a gene and/or altered expression of an miRNA, wherein the altered expression results in increased or decreased production of a virus. The virus is a picornavirus, such as a poliovirus or Enterovirus 71. Also provided herein are methods for using the engineered cells to produce virus, and methods for treating a subject having or at risk of having a viral infection.