公开(公告)号：EP1208217B1

公开(公告)日：2008-10-29

申请号：EP00963282.9

申请日：2000-08-30

申请人： THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL

发明人： MACDONALD, Gene, H. ,  JOHNSTON, Robert, E.

IPC分类号： C12N15/86 ,  C12N15/87 ,  A61K39/00

CPC分类号： C12N15/87 ,  A61K48/00 ,  A61K2039/5256 ,  C12N15/86 ,  C12N2770/36143

公开(公告)号：EP1208217A1

公开(公告)日：2002-05-29

申请号：EP00963282.9

申请日：2000-08-30

申请人： The University of North Carolina at Chapel Hill

发明人： MACDONALD, Gene, H. ,  JOHNSTON, Robert, E.

IPC分类号： C12N15/86 ,  C12N15/87 ,  A61K39/00

CPC分类号： C12N15/87 ,  A61K48/00 ,  A61K2039/5256 ,  C12N15/86 ,  C12N2770/36143

摘要： The present invention provides compositions and methods for delivering a nucleotide sequence to a cell using an alphavirus vector that is complexed with an enhancing antibody that specifically binds to the alphavirus vector. Venezuelan Equine Encephalitis vectors are preferred. The cell may be a cell in vitro or in vivo. Alternatively, the cell may be removed from a subject, administered the alphavirus vector ex vivo and then administered to a subject. Antigen-presenting cells are preferred, with dendritic cells being more preferred. Also provided are methods of producing an immune response in a subject, e.g., for producing an immune response against an antigen associated with a pathogen or for immunotherapy of cancer of tumors.