公开(公告)号：EP0252685B1

公开(公告)日：1993-06-16

申请号：EP87305863.0

申请日：1987-07-02

申请人： THE WISTAR INSTITUTE

发明人： Tsujimoto,Yoshihide ,  Croce,Carlos M

IPC分类号： G01N33/574 ,  C12Q1/68

CPC分类号： C07K14/82 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N33/5748 ,  Y10S436/813 ,  Y10S530/806 ,  Y10S530/828

公开(公告)号：EP0252685A3

公开(公告)日：1990-09-26

申请号：EP87305863.0

申请日：1987-07-02

申请人： THE WISTAR INSTITUTE

发明人： Tsujimoto,Yoshihide ,  Croce,Carlos M

IPC分类号： G01N33/574 ,  C12Q1/68

摘要： The sequence of the protein coding regions of the bcl-2 gene are provided as well as bacterial clones which produce the proteins. Assays are provided for detecting a class of B-cell neoplasms associated with a chromosome translocation between chromosomes 14 and 18. This translocation is involved in the majority of cases of human follicular lymphomas. One assay employs an antibody which is immunoreactive with a human protein which is over-expressed due to the chromosome translocation. Another assay involves measurement of the amount of mRNA which hybridizes to the gene proximal to the translocation break-point.

摘要翻译： 提供bcl-2基因的蛋白质编码区的序列以及产生蛋白质的细菌克隆。 提供了用于检测与染色体14和18之间的染色体易位有关的一类B细胞肿瘤的分析。这种易位涉及大多数人类滤泡性淋巴瘤病例。 一种测定使用与由于染色体易位而过表达的人蛋白质发生免疫反应的抗体。 另一种测定包括测量与易位断点附近的基因杂交的mRNA的量。

公开(公告)号：EP0252685A2

公开(公告)日：1988-01-13

申请号：EP87305863.0

申请日：1987-07-02

申请人： THE WISTAR INSTITUTE

发明人： Tsujimoto,Yoshihide ,  Croce,Carlos M

IPC分类号： G01N33/574 ,  C12Q1/68

CPC分类号： C07K14/82 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N33/5748 ,  Y10S436/813 ,  Y10S530/806 ,  Y10S530/828

摘要： The sequence of the protein coding regions of the bcl-2 gene are provided as well as bacterial clones which produce the proteins. Assays are provided for detecting a class of B-cell neoplasms associated with a chromosome translocation between chromosomes 14 and 18. This translocation is involved in the majority of cases of human follicular lymphomas. One assay employs an antibody which is immunoreactive with a human protein which is over-expressed due to the chromosome translocation. Another assay involves measurement of the amount of mRNA which hybridizes to the gene proximal to the translocation break-point.

摘要翻译： 提供bcl-2基因的蛋白质编码区的序列以及产生蛋白质的细菌克隆。 提供了用于检测与染色体14和18之间的染色体易位相关联的一类B细胞赘生物的测定。在大多数人卵泡淋巴瘤的病例中涉及这种易位。 一种测定采用与由于染色体易位而过度表达的人蛋白质具有免疫反应性的抗体。 另一种测定法包括测量与易位断裂点附近的基因杂交的mRNA的量。