公开(公告)号：EP2636735A4

公开(公告)日：2015-05-20

申请号：EP11832651

申请日：2011-10-13

申请人： TOKYO METROPOLITAN INST MEDICAL SCIENCE ,  CHEMO SERO THERAPEUT RES INST ,  UNIV HOKKAIDO NAT UNIV CORP

发明人： KOHARA MICHINORI ,  YASUI FUMIHIKO ,  MURAKAMI TOSHIO ,  KIDA HIROSHI ,  SAKODA YOSHIHIRO

IPC分类号： C12N15/09 ,  A61K31/711 ,  A61K35/76 ,  A61K39/145 ,  A61K48/00 ,  A61P31/16 ,  C12N7/04

CPC分类号： A61K39/145 ,  A61K31/711 ,  A61K39/12 ,  A61K2039/5256 ,  A61K2039/545 ,  C07K14/005 ,  C12N2710/24143 ,  C12N2760/16122 ,  C12N2760/16134

摘要： Provided are a highly-safe recombinant vaccinia virus that is effective in preventing the onset of symptoms due to infection by novel influenza viruses, and a vaccine for the novel influenza viruses containing the recombinant vaccinia virus. This recombinant vaccinia virus is capable of expressing the hemagglutinin protein genes of the novel influenza virus. This novel influenza vaccine contains the recombinant vaccinia virus.

公开(公告)号：EP2267120A4

公开(公告)日：2012-06-27

申请号：EP09717492

申请日：2009-03-06

申请人： TOKYO METROPOLITAN INST MEDICAL SCIENCE ,  CHEMO SERO THERAPEUT RES INST

发明人： KOHARA MICHINORI ,  MURAI FUKASHI

IPC分类号： C12N7/04 ,  A61K35/76 ,  A61P31/14 ,  C12N15/09

CPC分类号： C12N15/8636 ,  A01K67/0275 ,  A01K2217/15 ,  A01K2217/203 ,  A01K2267/0337 ,  A61K35/13 ,  A61K39/12 ,  A61K39/29 ,  A61K2039/525 ,  A61K2039/5256 ,  A61K2039/57 ,  C07K14/005 ,  C12N15/8509 ,  C12N2710/24143 ,  C12N2770/24222 ,  C12N2770/24234

公开(公告)号：EP2368989A4

公开(公告)日：2012-09-26

申请号：EP09829126

申请日：2009-11-26

申请人： CHUGAI PHARMACEUTICAL CO LTD ,  TOKYO METROPOLITAN INST MEDICAL SCIENCE

发明人： KOHARA MICHINORI ,  SUDO MASAYUKI

IPC分类号： C12N15/113 ,  A61K31/7088 ,  A61K31/713 ,  A61K35/76 ,  A61K48/00 ,  A61P31/14 ,  A61P31/16 ,  A61P31/18

CPC分类号： C12N15/113 ,  C12N15/111 ,  C12N15/1131 ,  C12N2310/14 ,  C12N2320/11

公开(公告)号：EP2842417A4

公开(公告)日：2016-01-06

申请号：EP13780731

申请日：2013-04-25

申请人： TOKYO METROPOLITAN INST MEDICAL SCIENCE ,  CHUGAI PHARMACEUTICAL CO LTD ,  PHOENIXBIO CO LTD

发明人： KOHARA MICHINORI ,  JISHAGE KOICHI ,  KAWASE YOSUKE ,  MUKAIDANI CHISE ,  OSHITA HIROKI ,  HAMAMURA SATOKO

IPC分类号： A01K67/027 ,  C12N15/09 ,  G01N33/15 ,  G01N33/50

CPC分类号： A01K67/0275 ,  A01K67/0271 ,  A01K2207/12 ,  A01K2207/15 ,  A01K2217/052 ,  A01K2217/15 ,  A01K2217/206 ,  A01K2227/105 ,  A01K2267/0337 ,  A01K2267/035 ,  C12N9/6462 ,  C12N15/8509 ,  C12N15/8775 ,  C12N2015/8581 ,  G01N33/5067 ,  G01N33/5088 ,  Y10T436/146666

摘要： The present invention provides a mouse with liver damage, having a high degree of damage against the mouse's original hepatocytes while having a uPA gene in a heterozygous form, and a method for efficiently preparing the mouse. Specifically, the method for preparing a mouse with liver damage having the uPA gene in a heterozygous form comprises the following steps of: (i) transforming mouse ES cells with a DNA fragment containing a liver-specific promoter/enhancer and cDNA that encodes a urokinase-type plasminogen activator operably linked under the control thereof; (ii) injecting the transformed mouse ES cells obtained in step (i) into a host embryo; (iii) transplanting the host embryo obtained in step (ii) via the injection of the ES cells into the uterus of a surrogate mother mouse, so as to obtain a chimeric mouse; and (iv) crossing the chimeric mice obtained in step (iii), so as to obtain a transgenic mouse in which the DNA fragment is introduced in a heterozygous form.

摘要翻译： 本发明提供了具有肝损伤的小鼠，其具有对小鼠原始肝细胞的高度损伤，同时具有杂合形式的uPA基因，以及有效制备小鼠的方法。 具体而言，制备具有uPA基因的肝损伤的小鼠具有杂合形式的方法包括以下步骤：（i）用含有肝特异性启动子/增强子的DNA片段和编码尿激酶的cDNA转化小鼠ES细胞 在其控制下可操作地连接的纤溶酶原激活剂; （ii）将步骤（i）中获得的转化的小鼠ES细胞注射到宿主胚胎中; （iii）通过将ES细胞注射到替代母亲小鼠的子宫中移植步骤（ii）中获得的宿主胚胎，从而获得嵌合小鼠; 和（iv）与步骤（iii）中获得的嵌合小鼠杂交，以获得其中DNA片段以杂合形式引入的转基因小鼠。

公开(公告)号：EP2471917A4

公开(公告)日：2013-03-13

申请号：EP10811924

申请日：2010-08-25

申请人： TOKYO METROPOLITAN INST MEDICAL SCIENCE ,  PHOENIXBIO CO LTD

发明人： KOHARA MICHINORI ,  ARAI MASAAKI ,  MUKAIDANI CHISE

IPC分类号： C12N15/09 ,  A01K67/027 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N7/00 ,  C12Q1/02 ,  G01N33/15 ,  G01N33/50

CPC分类号： C12Q1/707 ,  C12N7/00 ,  C12N2770/24221 ,  C12N2770/24222 ,  C12N2770/24243 ,  C12N2770/24251 ,  G01N2333/18 ,  G01N2500/10

摘要： A polynucleotide encoding the amino acid shown in SEQ ID NO:2 or SEQ ID NO: 5, or encoding an amino acid sequence having not less than 98% identity thereto; preferably a polynucleotide comprising replacement of the amino acid corresponding to glutamic acid at position 1202 of SEQ ID NO:2 (position 177 of SEQ ID NO:5) with glycine, replacement of the amino acid corresponding to glutamic acid at position 1056 (position 31 of SEQ ID NO:5) with valine, and replacement of the amino acid corresponding to alanine at position 2199 (position 1174 of SEQ ID NO:5) with threonine.