公开(公告)号：EP0667395A1

公开(公告)日：1995-08-16

申请号：EP94917140.9

申请日：1994-06-01

申请人： TORAY INDUSTRIES, INC.

发明人： NISHIKAWA, Satomi ,  SUDO, Tetsuo, Toray Industries Residence I-1 ,  OKANO, Kiyoshi ,  IZAWA, Akiko ,  NAKAMURA, Noriko ,  AKIYAMA, Naoko

IPC分类号： C12P21/08 ,  C07K15/28 ,  G01N33/577 ,  G01N33/53 ,  A01K67/027 ,  C12N5/06

CPC分类号： C07K16/2866 ,  A01K67/027 ,  A61K38/00

摘要： 5 n7 A monoclonal antibody specific for mouse IL-7 receptors, and an immunoassay method by which the receptors can be detected and determined readily with high sensitivity and accuracy. An artifically induced model prepared by administering the above antibody to an animal can be utilized in elucidating the mechanism of lymphocyte differentiation and proliferation, developing an immunosuppressant, and investigating organ transplantation, arthritis, diabetes, and so forth. Further, an artifically induced model prepared by administering the above antibody in combination with an anti-SCF receptor antibody to an animal can be utilized in elucidating diseases due to an abnormal hematopoietic function such as aplastic anemia exhibiting decreased myelopoiesis and whole blood hematopenia in the peripheral blood and in developing medicines capable of curing these blood cell disorders. A mouse containing the above antibody in combination with an anti-SCF receptor antibody administered thereto has concentrated hematopoietic stem cells, and hence the myeloma or splenic cells thereof can be used to estimate hematopoietic stem cell proliferation factors. Therefore, the invention method of estimation is useful in searching for such factors.

摘要翻译： 特异于小鼠IL-7受体的单克隆抗体，以及可以以高灵敏度和准确度容易地检测和确定受体的免疫测定方法。 通过向动物施用上述抗体而制备的人造诱发模型可用于阐明淋巴细胞分化和增殖的机制，开发免疫抑制剂，以及研究器官移植，关节炎，糖尿病等。 此外，通过将上述抗体与抗SCF受体抗体组合施用于动物而制备的专门诱导的模型可用于阐明由于造血功能异常引起的疾病，例如在外周期中显示骨髓细胞减少和全血血细胞减少的再生障碍性贫血 血液和开发能够治愈这些血细胞疾病的药物。 含有上述抗体与施用于其的抗SCF受体抗体组合的小鼠具有浓缩的造血干细胞，因此其骨髓瘤或脾细胞可用于估计造血干细胞增殖因子。 因此，本发明的估计方法在寻找这些因素方面是有用的。