公开(公告)号：EP3395943A1

公开(公告)日：2018-10-31

申请号：EP16878613.5

申请日：2016-12-19

Applicant: Public University Corporation Yokohama City University

Inventor： TANIGUCHI, Hideki ,  NIE, Yunzhong ,  ZHENG, Yun-Wen ,  SEKINE, Keisuke ,  TAKEBE, Takanori

IPC: C12N5/077 ,  A01K67/027 ,  C12N5/0775 ,  C12Q1/02

CPC classification number: A01K67/0271 ,  A01K67/027 ,  A01K2227/10 ,  A01K2267/025 ,  A01K2267/03 ,  C12N5/0062 ,  C12N5/0647 ,  C12N5/0677 ,  C12Q1/02

Abstract: A revised technique for preparing human tissues and organs aiming at application to medical use is provided. With this technique, function per cell is greatly improved and great reduction in production cost is realized.
A method of preparing an organ bud, comprising culturing vascular endothelial cells, mesenchymal cells and a tissue or organ cell in vitro in the presence of blood cells. Also provided are an organ bud prepared by the above method and a method of preparing a tissue or an organ using the organ bud. Using the same organ bud, there are provided a method of transplanting an organ bud, a method of regeneration or function recovery of a tissue or an organ, a method of preparing a non-human chimeric animal, and a method of evaluating a drug.

公开(公告)号：EP1965658B1

公开(公告)日：2018-10-03

申请号：EP06817510.8

申请日：2006-11-17

Applicant: Commonwealth Scientific and Industrial Research Organisation

Inventor： MILLER, Matthew, Robert ,  CARTER, Christopher, Guy ,  NICHOLS, Peter, David ,  SINGH, Surinder, Pal ,  ZHOU, Xue-Rong ,  GREEN, Allan, Graham

IPC: A23K20/158 ,  A23K50/80

CPC classification number: A23K20/158 ,  A01K61/10 ,  A01K61/80 ,  A01K67/027 ,  A01K2227/40 ,  A01K2227/70 ,  A01K2267/02 ,  A23K20/142 ,  A23K20/147 ,  A23K50/10 ,  A23K50/75 ,  A23K50/80 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/8247 ,  C12P7/6472 ,  Y02A40/81 ,  Y02A40/818 ,  Y02A40/845 ,  Y10S426/805

Abstract: The present invention relates to feedstuffs for use in aquaculture, as well as methods for producing said feedstuffs. The invention also provides methods for rearing fish and/or crustaceans. In particular, the present invention provides a method of rearing a fish or crustacean, the method comprising feeding the fish or crustacean a feedstuff comprising lipid, the fatty acid of said lipid comprising at least 5.5% (w/w) stearidonic acid (SDA).

公开(公告)号：EP2675271B1

公开(公告)日：2018-06-13

申请号：EP12746594.6

申请日：2012-02-14

Applicant: Regeneron Pharmaceuticals, Inc. ,  Yale University ,  Institute for Research in Biomedicine (IRB)

Inventor： MURPHY, Andrew J. ,  STEVENS, Sean ,  RATHINAM, Chozhavendan ,  EYNON, Elizabeth ,  MANZ, Markus G. ,  FLAVELL, Richard ,  YANCOPOULOS, George D.

IPC: A01K67/027

CPC classification number: A01K67/0278 ,  A01K67/027 ,  A01K67/0271 ,  A01K2207/12 ,  A01K2207/15 ,  A01K2217/072 ,  A01K2217/15 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0337 ,  A61K49/0008 ,  C12N15/8509 ,  C12N2015/8536 ,  G01N33/5088 ,  G01N2500/10

Abstract: Genetically modified mice comprising a nucleic acid sequence encoding a human M-CSF protein are provided. Also provided are genetically modified mice comprising a nucleic acid sequence encoding a human M-CSF protein that have been engrafted with human cells such as human hematopoietic cells, and methods for making such engrafted mice. These mice find use in a number of applications, such as in modeling human immune disease and pathogen infection; in in vivo screens for agents that modulate hematopoietic cell development and/or activity, e.g. in a healthy or a diseased state; in in vivo screens for agents that are toxic to hematopoietic cells; in in vivo screens for agents that prevent against, mitigate, or reverse the toxic effects of toxic agents on hematopoietic cells; in in vivo screens of human hematopoietic cells from an individual to predict the responsiveness of an individual to a disease therapy, etc.

公开(公告)号：EP3222718A4

公开(公告)日：2018-05-30

申请号：EP15861997

申请日：2015-11-17

Applicant: UNIV KYOTO

Inventor： MASHIMO TOMOJI ,  YOSHIMI KAZUTO ,  KANEKO TAKEHITO

IPC: C12N15/09 ,  A01K67/027 ,  C12N5/10 ,  C12N15/90

CPC classification number: A01K67/0278 ,  A01K67/027 ,  A01K2207/15 ,  A01K2217/052 ,  A01K2217/072 ,  A01K2227/105 ,  C12N5/10 ,  C12N15/09 ,  C12N15/907

Abstract: This invention provides a method for knock-in of a donor DNA into the genome of a cell, comprising introducing at least one artificial nuclease system capable of cleaving target sequence(s) of the cell genome, the donor DNA, and two single-stranded oligonucleotides (ssODNs) into the cell, the artificial nuclease system cleaving the target sequence(s) on the cell genome, the two ssODNs each complementary to one of the ends generated by the target sequence cleavage in the cell genome and to one of the introduction ends of the donor DNA, the donor DNA being knocked-in at the cleavage site via the two ssODNs.

公开(公告)号：EP2623106B1

公开(公告)日：2018-05-23

申请号：EP10857831.1

申请日：2010-09-29

Applicant: Shiseido Company, Ltd.

Inventor： YOSHIDA, Yuzo ,  SOMA, Tsutomu ,  FUJIWARA, Shigeyoshi

IPC: C12N5/071 ,  A61K35/36 ,  A61P17/14

CPC classification number: A01K67/027 ,  A01K67/0271 ,  A01K2207/12 ,  A01K2227/105 ,  A01K2267/03 ,  A61K8/985 ,  A61K35/36 ,  A61K2800/70 ,  A61L27/3813 ,  A61L2430/18 ,  A61Q7/00 ,  C12N5/0627 ,  C12N5/0628 ,  C12N5/0666

Abstract: A composition for regenerating the follicle, which contains CD36-expressing connective tissue sheath cells (DSc).

公开(公告)号：EP2635110B1

公开(公告)日：2018-05-09

申请号：EP11838895.8

申请日：2011-11-04

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： DEISSEROTH, Karl ,  SOHAL, Vikaas ,  GUNAYDIN, Lisa

IPC: G01N33/50 ,  A01K67/00

CPC classification number: A01K67/0278 ,  A01K67/027 ,  A01K67/0275 ,  A01K2217/052 ,  A01K2227/105 ,  A01K2267/0393 ,  A61K48/005 ,  A61K48/0075 ,  A61K49/00 ,  A61K49/0004 ,  A61N5/0618 ,  C12N5/0619 ,  G01N33/5058 ,  G01N33/5088

Abstract: Provided herein are methods of inducing psychosis in animals using light-responsive opsins and methods of identifying or screening compounds that may be useful in treating psychosis.

公开(公告)号：EP3311660A1

公开(公告)日：2018-04-25

申请号：EP16814302.2

申请日：2016-06-20

Applicant: National Federation of Agricultural Cooperative Associations ,  The University Of Tokyo

Inventor： FUKAWA, Kazuo ,  ITO, Tetsuya ,  KAMIKAWA, Mai ,  SENDAI, Yutaka ,  NAKAUCHI, Hiromitsu ,  YAMAZAKI, Satoshi ,  WATANABE, Motoo

IPC: A01K67/027 ,  C12N5/16 ,  C12N15/09

CPC classification number: A01K67/0271 ,  A01K67/027 ,  A01K2207/12 ,  A01K2217/052 ,  A01K2227/108 ,  A01K2267/025 ,  C12N5/0647 ,  C12N15/09 ,  C12N2510/00 ,  C12N2510/02 ,  C12N2517/02

Abstract: The present invention discloses a novel means capable of producing a blood chimeric animal in which a state of retaining blood cells originating in a heterologous animal, such as a human, at a high percentage is sustained for a long period of time, even in cases where a medium to large domestic animal is used as a recipient. The method for producing a non-human animal that retains blood cells originating in a heterologous animal, according to the present invention, comprises transplanting hematopoietic cells of a heterologous animal into a non-human animal, in which hematopoietic cells the function of a gene that acts on the hematopoietic system is modified. The gene that acts on the hematopoietic system is, for example, Lnk gene. According to the method of the present invention, when a medium to large mammal such as a pig is used as a recipient, the survival rate of hematopoietic cells originating in a heterologous animal is dramatically increased such that blood chimerism of 10% or more can be maintained even in a 16 month old animal.

Abstract translation: 本发明公开了一种能够制造血液嵌合体动物的新型方法，其中以高百分比保存源自异源动物如人的血细胞的状态持续很长一段时间，即使在 一个大型的家养动物被用作接受者。 根据本发明的用于生产保留源自异源动物的血细胞的非人动物的方法包括将异源动物的造血细胞移植到非人动物中，其中造血细胞具有基因的功能， 对造血系统的作用被修改。 作用于造血系统的基因例如是Lnk基因。 根据本发明的方法，当使用猪等大型哺乳动物作为受体时，源自异源动物的造血细胞的存活率显着增加，使得10％或更多的血液嵌合体可以是 即使在16个月大的动物中也能保持。

公开(公告)号：EP3297429A2

公开(公告)日：2018-03-28

申请号：EP16725715.3

申请日：2016-05-17

Applicant: University of Maryland, Baltimore County

Inventor： WONG, Ten, Tsao ,  ZOHAR, Yonathan

IPC: A01K67/027 ,  C12N15/87

CPC classification number: A01K67/0275 ,  A01K67/027 ,  A01K2207/05 ,  A01K2207/20 ,  A01K2227/40 ,  A01K2267/02 ,  A01K2267/03 ,  C12N15/113 ,  C12N15/87 ,  C12N2310/11 ,  C12N2310/3233 ,  C12N2310/351 ,  C12N2320/32 ,  Y02A90/40

Abstract: Methods are provided for delivery of at least one agent into egg(s) from an egg-producing aquatic animal including contacting fertilized or unfertilized egg(s) from said egg-producing aquatic animal with the at least one agent in the presence of a guanidine-containing compound capable of enhancing the permeability of the chorion of the egg(s). Methods are provided for the drug screening and compound toxicity assays. Methods are also provided for the production of reproductively sterile fish and aquatic animals for aquaculture, the aquarium trade, and control of invasive species are described. The methods include disruption of gonadal development through the administration of agents that lead to the failure of fertile gonadal development. Agents may be delivered to the eggs directly before the fertilization or post fertilization by contacting eggs in an immersion medium including the agent of interest.

公开(公告)号：EP2343080B1

公开(公告)日：2017-11-08

申请号：EP09817788.4

申请日：2009-09-30

Applicant: Kyowa Hakko Kirin Co., Ltd.

Inventor： KAKITANI, Makoto ,  TOMIZUKA, Kazuma

IPC: A61K38/00 ,  A61K48/00 ,  A61P19/08 ,  A61P19/10

CPC classification number: A61K38/177 ,  A01K67/027 ,  A01K67/0271 ,  A01K2207/12 ,  A01K2207/30 ,  A01K2217/072 ,  A01K2227/105 ,  A01K2267/035 ,  A61K31/7088 ,  A61K38/1709 ,  A61K39/3955 ,  A61K45/06 ,  A61K47/60 ,  A61K47/6835 ,  A61K48/00 ,  C07K14/723 ,  C07K2319/30

Abstract: This invention relates to a pharmaceutical composition for treatment of a bone disease comprising, as an active ingredient, a protein comprising an extracellular cysteine-rich domain, which is from the Frizzled receptor selected from the group consisting of mammalian animal-derived Frizzled 1, Frizzled 2, and Frizzled 7 and has activity of increasing bone mass, bone density, and/or bone strength, or a mutant of such domain having sequence identity of 85% or higher to the amino acid sequence of the domain and having activity of increasing bone mass, bone density, and/or bone strength, or a vector comprising a nucleic acid encoding the protein.

公开(公告)号：EP3226853A1

公开(公告)日：2017-10-11

申请号：EP15816590.2

申请日：2015-12-01

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： YUAN, Ming ,  HU, Ying ,  CAO, Jingtai ,  ROMANO, Carmelo

IPC: A61K31/14 ,  A61K31/46 ,  A61P27/02

CPC classification number: A01K67/027 ,  A01K2207/20 ,  A01K2227/10 ,  A01K2227/105 ,  A01K2267/03 ,  A61K31/14 ,  A61K31/46 ,  A61K49/0008

Abstract: Provided herein are methods for creating an animal model for the study of dry eye and methods of using such animal models to test candidate treatments for ocular conditions and determine the efficacy of such treatments.

Abstract translation: 本文提供了用于创建用于干眼研究的动物模型的方法以及使用这种动物模型来测试候选眼用条件处理并确定这些处理的功效的方法。