公开(公告)号：EP1309340A4

公开(公告)日：2005-05-18

申请号：EP01964176

申请日：2001-08-17

申请人： TRANSKARYOTIC THERAPIES INC

发明人： KINOSHITA CAROL A ,  PRASHSANT MISHRA ,  BOROWSKI MARIANNE ,  FRANCIS-DANIEL PETER

IPC分类号： C12N15/09 ,  A61K38/43 ,  A61K38/47 ,  A61P3/06 ,  C12N5/10 ,  C12N9/24 ,  C12N9/99 ,  C12N15/56 ,  C12P21/00 ,  C07D211/46 ,  C12N9/42 ,  C12N15/85

CPC分类号： C12Y302/01045 ,  A61K38/47 ,  C12N9/2402 ,  C12P21/005 ,  C12Y302/01024

摘要： The invention features a method of producing a high mannose glucocerebrosidase (hmGCB) which includes: providing a cell which is capable of expressing glucocerebrosidase (GCB), and allowing production of GCB having a precursor oligosaccharide under conditions which prevent the removal of at least one mannose residue distal to the pentasaccharide core of the precursor oligosaccharide of GCB, to thereby produce an hmGCB preparation. Preferably, the condition which prevents the removal of at least one mannose residue distal to the pentasaccharide core is inhibition of a class 1 processing mannosidase and/or a class 2 processing manosidase. The invention also features an hmGCB preparation and methods of using an hmGCB preparation.