公开(公告)号：EP1225229A1

公开(公告)日：2002-07-24

申请号：EP00969984.4

申请日：2000-10-23

申请人： Takara Shuzo Co, Ltd.

发明人： UENO, Takashi, 609, Hamoparesu-Kusatsu ,  MATSUMURA, Hajime ,  TANAKA, Keiji ,  IWASAKI, Tomoko ,  UENO, Mitsuhiro ,  FUJINAGA, Kei ,  ASADA, Kiyozo ,  KATO, Ikunoshin

IPC分类号： C12N15/861 ,  A61K35/12 ,  A61K35/76 ,  A61K48/00 ,  C12N5/10 ,  C12N7/01

CPC分类号： C12N15/86 ,  C12N2710/10343 ,  C12N2800/30

摘要： A method of transferring a foreign gene into cells, characterized by involving: the step of transferring into the cells with the use of an adenovirus vector, a first nucleic acid, which has a sequence provided with adeno-associated virus-origin ITRs in both sides of the target foreign gene to be transferred, and a second nucleic acid, which has an adeno-associated virus-origin rep gene and a promoter for expressing this gene and carries a stuffer sequence inserted thereinto sandwiched in two recombinase recognition sequences and located between the rep gene and the promoter; and the step of expressing the Rep protein under the action of recombinase in the cells obtained in the above step to thereby integrate the target foreign gene into the chromosomal DNA.

摘要翻译： 将外源基因转移到细胞中的方法，其特征在于包括：使用腺病毒载体转移到细胞中的步骤，第一核酸，其具有在两侧具有腺相关病毒起源ITR的序列 的待转移的靶外源基因的第二核酸和具有腺相关病毒起源rep基因的第二核酸和用于表达该基因并携带插入其中的填充序列的第二核酸夹在两个重组酶识别序列中并位于 rep基因和启动子; 以及在上述步骤中获得的细胞中在重组酶的作用下表达Rep蛋白的步骤，从而将靶外源基因整合到染色体DNA中。