公开(公告)号：EP3227462B1

公开(公告)日：2020-04-22

申请号：EP15865948.2

申请日：2015-12-01

申请人： The Broad Institute, Inc. ,  Baylor College of Medicine

发明人： LIEBERMAN-AIDEN, Erez ,  RAO, Suhas ,  STAMENOVA, Elena ,  DUDCHENKO, Olga ,  LANDER, Eric

IPC分类号： C12Q1/6806 ,  C12Q1/6869 ,  C40B20/00

公开(公告)号：EP3227462A1

公开(公告)日：2017-10-11

申请号：EP15865948.2

申请日：2015-12-01

申请人： The Broad Institute Inc. ,  Baylor College of Medicine

发明人： LIEBERMAN-AIDEN, Erez ,  RAO, Suhas ,  STAMENOVA, Elena ,  DUDCHENKO, Olga ,  LANDER, Eric

IPC分类号： C12Q1/68 ,  C40B20/00 ,  C40B30/04

CPC分类号： C12Q1/6869 ,  C12Q1/6806 ,  C12Q2521/501 ,  C12Q2525/117 ,  C12Q2535/122 ,  C12Q2563/131 ,  C40B20/00 ,  C40B30/04

摘要： Disclosed is an in situ method for detecting spatial proximity relationships between nucleic acid sequences, such as DNA, in a cell. The method includes: providing a sample of one or more cells comprising nucleic acids; fragmenting the nucleic acids present in the cells that leaves 5′ overhanging ends; filling in the overhanging ends with at least one labeled nucleotide; joining the filled in end of the fragmented nucleic acids that are in close physical proximity to create one or more end joined nucleic acid fragments having a junction; isolating the one or more end joined nucleic acid fragments using the labeled nucleotide; and determining the sequence at the junction of the one or more end joined nucleic acid fragments.

摘要翻译： 公开了用于检测细胞中的核酸序列例如DNA之间的空间邻近关系的原位方法。 该方法包括：提供一个或多个包含核酸的细胞的样品; 将存在于存在于5'突出末端的细胞中的核酸片段化; 用至少一个标记的核苷酸填充突出端; 连接紧密物理接近的片段化核酸的填充末端以产生具有连接处的一个或多个末端连接的核酸片段; 使用标记的核苷酸分离一个或多个末端连接的核酸片段; 并确定一个或多个末端连接的核酸片段连接处的序列。