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公开(公告)号：EP3227462B1
公开(公告)日：2020-04-22
申请号：EP15865948.2
申请日：2015-12-01
IPC分类号： C12Q1/6806 , C12Q1/6869 , C40B20/00
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2.发明公开
公开(公告)号：EP3337908A1
公开(公告)日：2018-06-27
申请号：EP16837870.1
申请日：2016-08-18
申请人： The Broad Institute, Inc. , Baylor College of Medicine , Lieberman Aiden, Erez , Rao, Suhas , Huang, Su-chen , Sanborn, Adrian L. , Durand, Neva C. , Huntley, Miriam , Jewett, Andrew
发明人： LIEBERMAN AIDEN, Erez , LANDER, Eric, S. , RAO, Suhas , HUANG, Su-Chen , SANBORN, Adrian, L. , DURAND, Neva, C. , HUNTLEY, Miriam , JEWETT, Andrew
CPC分类号： C12N15/113 , C07K14/47 , C07K14/4702 , C12N15/11 , C12N2310/14 , C12N2310/20
摘要： Chromatin 3D structure modulating agents in the context of the present invention are intended to interfere or manipulate the function of loop anchor motifs, such as CTCF motifs. In certain example embodiments, the present invention may block formation of an loop anchor or chromatin domain or induce formation of a loop anchor or chromatin domain at a targeted genomic location. For instance, a loop anchor motif can be altered, such as by mutating (including inverting) a binding motif so as to remove such a motif, or by adding new binding motifs in new locations within a loop domain, so as to reduce the size of an existing loop, so as to modify the size of an existing loop, or combinations thereof. Alternatively, the chromatin 3D structure modulating agent may bind a target region and mask a loop anchor motif, thereby preventing a loop anchor or chromatin domain from forming. The chromatin 3D structure modulating agent may bind a target region and cause a loop anchor of chromatin domain to form.
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公开(公告)号：EP3227462A1
公开(公告)日：2017-10-11
申请号：EP15865948.2
申请日：2015-12-01
CPC分类号： C12Q1/6869 , C12Q1/6806 , C12Q2521/501 , C12Q2525/117 , C12Q2535/122 , C12Q2563/131 , C40B20/00 , C40B30/04
摘要： Disclosed is an in situ method for detecting spatial proximity relationships between nucleic acid sequences, such as DNA, in a cell. The method includes: providing a sample of one or more cells comprising nucleic acids; fragmenting the nucleic acids present in the cells that leaves 5′ overhanging ends; filling in the overhanging ends with at least one labeled nucleotide; joining the filled in end of the fragmented nucleic acids that are in close physical proximity to create one or more end joined nucleic acid fragments having a junction; isolating the one or more end joined nucleic acid fragments using the labeled nucleotide; and determining the sequence at the junction of the one or more end joined nucleic acid fragments.
摘要翻译： 公开了用于检测细胞中的核酸序列例如DNA之间的空间邻近关系的原位方法。 该方法包括：提供一个或多个包含核酸的细胞的样品; 将存在于存在于5'突出末端的细胞中的核酸片段化; 用至少一个标记的核苷酸填充突出端; 连接紧密物理接近的片段化核酸的填充末端以产生具有连接处的一个或多个末端连接的核酸片段; 使用标记的核苷酸分离一个或多个末端连接的核酸片段; 并确定一个或多个末端连接的核酸片段连接处的序列。
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4.发明公开METHODS FOR DETERMINING MULTIPLE INTERACTIONS BETWEEN NUCLEIC ACIDS IN A CELL 审中-公开用于确定NUCLEIC之间的多重相互作用在细胞
公开(公告)号：EP2971278A1
公开(公告)日：2016-01-20
申请号：EP14764060.1
申请日：2014-03-14
IPC分类号： C40B30/00
CPC分类号： C12Q1/6883 , C12Q1/68 , C12Q1/6876 , C12Q2600/158 , C12Q2565/133
摘要： Disclosed are methods for detecting spatial proximity relationships between nucleic acid sequences in a cell. The methods include: providing a sample of one or more cells comprising nucleic acids; fragmenting the nucleic acids present in the cells, wherein the fragmented nucleic acids have ends capable of joining to other fragmented nucleic acids; joining ends of fragmented nucleic acids to other ends fragmented nucleic acid to create at least one nucleic acid concatemer having at least one junction between the joined fragmented nucleic acids, and wherein the at least one nucleic acid concatemer encodes the information about the proximity of the DNA sequences in the cell; and determining the sequence at least one junction of the at least one nucleic acid concatemer, thereby detecting spatial proximity relationships between nucleic acid sequences in a cell.
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