公开(公告)号：EP0399681B1

公开(公告)日：2000-01-19

申请号：EP90304796.7

申请日：1990-05-02

申请人： The Clorox Company

发明人： El-Sayed, Maha Y. ,  Lewis, Sheldon N. ,  Anderson, Susan A. ,  Wiersema, Richard J.

IPC分类号： C11D3/386

CPC分类号： C11D3/38636 ,  C11D3/38627

公开(公告)号：EP0359087A3

公开(公告)日：1991-10-23

申请号：EP89116326.3

申请日：1989-09-05

申请人： The Clorox Company

发明人： Stanislowski, Anna G. ,  Heitfeld, Fred A. ,  Wiersema, Richard J. ,  Poulose, Ayrookaram J.

IPC分类号： C11D3/386 ,  C11D3/39

CPC分类号： C11D3/386 ,  C11D3/391

摘要： A perhydrolysis system or activated oxidant system for in situ generation of peracid in aqueous solutions is disclosed, including: (a) a protease enzyme; (b) an ester substrate having the structure
wherein R = C 1-11 -alkyl, R = C 1-10 -alkyl or H, Z = 0 or
a polyalkoxy residue of the formula (CH 2 CH 2 0) m or
wherein m = 0-10, r=0 or 1; R and R optionally having OH-substituents pendant on or terminating the hydrocarbyl chains, and (c) a source of peroxygen capable of reacting with the protease (a) and the ester substrate (b) to result in proteolytic production of of peracid.

公开(公告)号：EP0476915A2

公开(公告)日：1992-03-25

申请号：EP91308282.2

申请日：1991-09-11

申请人： The Clorox Company

发明人： Mizusawa, Eugene A. ,  Anderson, Susan A. ,  El-Sayed, Maha Y. ,  Leiske, Daniel R. ,  Wiersema, Richard J. ,  Yang, Chihae

IPC分类号： C11D3/386 ,  D06M16/00

CPC分类号： C11D3/38627 ,  D06M16/003 ,  Y10S435/877

摘要： Methods for treating surfaces with lipase to provide an enzyme-surface complex and surfaces so treated facilitate oil removal. One such treated surface is a fabric with lipase sorbed onto the surface. A preferred sorbed lipase is isolatable from a Pseudomonas organism or clone. Treated fabrics have substantial hydrolysis activity for oil stains and perhydrolytic activity for oil stains, have altered surface wettability, and retard oil and hydrolysis by-produce redisposition in the presence of aqueous solutions. The sorbed lipase is resistant to removal during fabric laundering and retains substantial hydrolytic activity even with exposure to drying at elevated temperature. The hydrolytic activity of the lipase-fabric complex persists during storage or wear. Hydrolysis by-products are removable during laundering at basic pH or in the presence of surfactant.

摘要翻译： 用脂肪酶处理表面以提供酶表面复合物和如此处理的表面的方法促进除油。 一个这样处理的表面是具有吸附在表面上的脂肪酶的织物。 优选的吸附脂肪酶可与假单胞菌生物体或克隆分离。 经处理的织物对油渍具有显着的水解活性和油渍的过水解活性，具有改变的表面润湿性，并且在水溶液存在下延迟油和水解副产物再分配。 吸收的脂肪酶在织物洗涤期间耐除去，并且即使在高温下暴露于干燥中也能保持显着的水解活性。 脂肪酶织物复合物的水解活性在储存或磨损期间仍然存在。 水解副产物在碱性pH下或在表面活性剂存在下洗涤时可以除去。

公开(公告)号：EP0399681A2

公开(公告)日：1990-11-28

申请号：EP90304796.7

申请日：1990-05-02

申请人： The Clorox Company

发明人： El-Sayed, Maha Y. ,  Lewis, Sheldon N. ,  Anderson, Susan A. ,  Wiersema, Richard J.

IPC分类号： C11D3/386

CPC分类号： C11D3/38636 ,  C11D3/38627

摘要： The activation of glycerol ester hydrolases, toward triglyceride oils, is correlated to the molar ratio of total triglyceride to surfactant concentrations in the test solution. This ratio dependency, when considered in laundry compositions, allows for predictable and improved utilization of these enzymes in the hydrolysis of triglyceride stains. A particularly preferred enzyme for this use is isolatable from Pseudomonas putida ATCC 53552. This enzyme, or hydrolase, has sufficient hydrolysis activity in a laundering solution to hydrolyze at least about 5 wt. % of total triglyceride stains in a laundering solution within about 15 minutes at about 25°C.

摘要翻译： 甘油酯水解酶对甘油三酯油的活化与测试溶液中总甘油三酯与表面活性剂浓度的摩尔比相关。 当在洗衣组合物中考虑时，这种比例依赖性允许在甘油三酯污渍的水解中这些酶的可预测和改进的利用。 用于此用途的特别优选的酶可从恶臭假单胞菌ATCC 53552分离。该酶或水解酶在洗涤溶液中具有足够的水解活性以水解至少约5wt。 在约25℃下约15分钟内洗涤溶液中总甘油三酯染色的百分比

公开(公告)号：EP0359087A2

公开(公告)日：1990-03-21

申请号：EP89116326.3

申请日：1989-09-05

申请人： The Clorox Company

发明人： Stanislowski, Anna G. ,  Heitfeld, Fred A. ,  Wiersema, Richard J. ,  Poulose, Ayrookaram J.

IPC分类号： C11D3/386 ,  C11D3/39

CPC分类号： C11D3/386 ,  C11D3/391

摘要： A perhydrolysis system or activated oxidant system for in situ generation of peracid in aqueous solutions is disclosed, including: (a) a protease enzyme; (b) an ester substrate having the structure


wherein R = C 1-11 -alkyl, R = C 1-10 -alkyl or H,
Z = 0 or

a polyalkoxy residue of the formula (CH 2 CH 2 0) m or


wherein m = 0-10,
r=0 or 1;
R and R optionally having OH-substituents pendant on or terminating the hydrocarbyl chains, and (c) a source of peroxygen capable of reacting with the protease (a) and the ester substrate (b) to result in proteolytic production of of peracid.

摘要翻译： 公开了用于在水溶液中原位产生过酸的过水解系统或活化氧化剂系统，包括：（a）蛋白酶; （b）具有结构R min（Z）@（CH 2） - -OR的酯底物，其中R = C 1-11烷基，R min = C 1-10 - 烷基或H，Z = O或多烷氧基残基 式（CH 2 CH 2 O）m或其中m = 0-10，r = 0或1; R和R min任选地具有挂在烃基链上或端基上的OH取代基，和（c）能够与蛋白酶（a）和酯底物（b）反应的过氧源，导致过酸的蛋白水解产生。

公开(公告)号：EP0268456A3

公开(公告)日：1989-04-26

申请号：EP87310130.7

申请日：1987-11-17

申请人： The Clorox Company

发明人： Wiersema, Richard J. ,  Stanislowski, Anna G. ,  Gray, Gregory L. ,  Poulose, Ayrookaram J. ,  Power, Scott D.

IPC分类号： C11D3/386 ,  C12N9/20

CPC分类号： C11D3/3945 ,  C11D3/2093 ,  C11D3/38627 ,  C11D3/39 ,  C11D3/391 ,  C12N9/20 ,  D06L4/40

摘要： An enzymatic perhydrolysis system, useful for bleaching, has a novel enzyme, a substrate, and a source of hydrogen peroxide, and provides in situ formation of peracid in aqueous solution. The substrate is selected for enzyme catalyzed reaction, and preferably is an acylglycerol with two or three fatty acid chains. The enzyme is hydrolytically and perhydrolytically active even in the presence of anionic surfactants, has lipase acitivity, and is isolatable from Pseudomonas putida ATCC 53552.

摘要翻译： 用于漂白的酶促全水解系统具有新型酶，底物和过氧化氢源，并且在水溶液中原位形成过酸。 选择底物用于酶催化反应，并且优选是具有两个或三个脂肪酸链的酰基甘油。 该酶即使在阴离子表面活性剂存在下也具有水解和水解活性，具有脂肪酶活性，并且可从恶臭假单胞菌ATCC 53552分离。

公开(公告)号：EP0476915B1

公开(公告)日：1997-05-14

申请号：EP91308282.2

申请日：1991-09-11

申请人： The Clorox Company

发明人： Mizusawa, Eugene A. ,  Anderson, Susan A. ,  El-Sayed, Maha Y. ,  Leiske, Daniel R. ,  Wiersema, Richard J. ,  Yang, Chihae

IPC分类号： C11D3/386 ,  D06M16/00

CPC分类号： C11D3/38627 ,  D06M16/003 ,  Y10S435/877

公开(公告)号：EP0390393B1

公开(公告)日：1995-05-10

申请号：EP90302949.4

申请日：1990-03-19

申请人： The Clorox Company

发明人： Rowland, Richard R. ,  Wiersema, Richard J. ,  Fong, Ronald A. ,  Zielske, Alfred G.

IPC分类号： C11D3/39 ,  C07C69/675 ,  C07C409/24

CPC分类号： C11D3/3907

公开(公告)号：EP0399681A3

公开(公告)日：1991-01-16

申请号：EP90304796.7

申请日：1990-05-02

申请人： The Clorox Company

发明人： El-Sayed, Maha Y. ,  Lewis, Sheldon N. ,  Anderson, Susan A. ,  Wiersema, Richard J.

IPC分类号： C11D3/386

CPC分类号： C11D3/38636 ,  C11D3/38627

摘要： The activation of glycerol ester hydrolases, toward triglyceride oils, is correlated to the molar ratio of total triglyceride to surfactant concentrations in the test solution. This ratio dependency, when considered in laundry compositions, allows for predictable and improved utilization of these enzymes in the hydrolysis of triglyceride stains. A particularly preferred enzyme for this use is isolatable from Pseudomonas putida ATCC 53552. This enzyme, or hydrolase, has sufficient hydrolysis activity in a laundering solution to hydrolyze at least about 5 wt. % of total triglyceride stains in a laundering solution within about 15 minutes at about 25°C.

公开(公告)号：EP0253487A2

公开(公告)日：1988-01-20

申请号：EP87304963.9

申请日：1987-06-04

申请人： The Clorox Company

发明人： Wiersema, Richard J. ,  Stanislowski, Anna G.

IPC分类号： C11D3/39 ,  C11D3/386

CPC分类号： C11D3/3945 ,  C11D3/2093 ,  C11D3/38627 ,  C11D3/39 ,  C11D3/391 ,  C12N9/20

摘要： A perhydrolysis system or activated oxidant system for in situ generation of peracid in aqueous solutions includes an esterase or lipase enzyme, a source of hydrogen peroxide and a functionalized ester substrate having the structure
wherein R is a substituent having at least one carbon atom and X is a functional moiety or group. Preferred substrates include glycerides, ethylene glycol derivatives and propylene glycol derivatives. The system is adapted for use in both high and low temperature wash conditions. In one embodiment, a lipase enzyme is employed with an insoluble substrate and an amulsify- ing agent. In another embodiment, an esterase and/or lipase enzyme, a glyceride substrate and hydrogen peroxide produce a peracid with active oxygen from a peracid of at least about 0. 5 ppm. Bleaching processes and stain removal capabilities are also disclosed.

摘要翻译： 用于在水溶液中原位产生过酸的过水解体系或活化氧化剂体系包括酯酶或脂肪酶，过氧化氢源和具有结构R 1 - O-CH 2 X的官能化酯底物，其中R是具有 至少一个碳原子并且是官能部分或基团。 优选的底物包括甘油酯，乙二醇衍生物和丙二醇衍生物。 该系统适用于高温和低温洗涤条件，在一个实施方案中，脂肪酶与不溶性底物和乳化剂一起使用，在另一个实施方案中，酯酶和/或脂肪酶，甘油酯底物和氢 过氧化物从至少约0.5ppm的过酸产生具有活性氧的过酸。 漂白工艺和去污能力也被披露。