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公开(公告)号：EP3192369B1
公开(公告)日：2018-12-12
申请号：EP16202467.3
申请日：2008-04-14
IPC分类号： A01N1/00
CPC分类号： A61F2/06 , A61F2/022 , A61F2/04 , A61F2/062 , A61F2002/068 , G01N33/5008 , G06F19/00
摘要： The invention provides method of fabricating a scaffold comprising a fluidic network, including the steps of: (a) generating an initial vascular layer for enclosing the chamber and providing fluid to the cells, the initial vascular layer having a network of channels for fluid; (b) translating the initial vascular layer into a model for fluid dynamics analysis; (c) analyzing the initial vascular layer based on desired parameters selected from the group consisting of a characteristic of a specific fluid, an input pressure, an output pressure, an overall flow rate and combinations thereof to determine sheer stress and velocity within the network of channels; (d) measuring the sheer stress and the velocity and comparing the obtained values to predetermined values; (e) determining if either of the shear stress or the velocity are greater than or less than the predetermined values, and (f) optionally modifying the initial vascular layer and repeating steps (b) - (e). The invention also provides compositions comprising a vascular layer for use in tissue lamina as well as a medical devices having a vascular layer and kits.
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2.发明授权
公开(公告)号：EP2154955B1
公开(公告)日：2016-12-07
申请号：EP08742923.9
申请日：2008-04-14
IPC分类号： A61F2/06
CPC分类号： A61F2/06 , A61F2/022 , A61F2/04 , A61F2/062 , A61F2002/068 , G01N33/5008 , G06F19/00
摘要： The invention provides method of fabricating a scaffold comprising a fluidic network, including the steps of: (a) generating an initial vascular layer for enclosing the chamber and providing fluid to the cells, the initial vascular layer having a network of channels for fluid; (b) translating the initial vascular layer into a model for fluid dynamics analysis; (c) analyzing the initial vascular layer based on desired parameters selected from the group consisting of a characteristic of a specific fluid, an input pressure, an output pressure, an overall flow rate and combinations thereof to determine sheer stress and velocity within the network of channels; (d) measuring the sheer stress and the velocity and comparing the obtained values to predetermined values; (e) determining if either of the shear stress or the velocity are greater than or less than the predetermined values, and (f) optionally modifying the initial vascular layer and repeating steps (b) - (e). The invention also provides compositions comprising a vascular layer for use in tissue lamina as well as a medical devices having a vascular layer and kits.
摘要翻译： 本发明提供了制造包括流体网络的支架的方法，包括以下步骤：（a）产生用于封闭腔室并向细胞提供流体的初始血管层，初始血管层具有用于流体的通道网络; （b）将初始血管层转化为用于流体动力学分析的模型; （c）基于从由特定流体的特性，输入压力，输出压力，总体流速及其组合组成的组中选择的期望参数来分析初始血管层，以确定网络内的纯粹的应力和速度 渠道; （d）测量纯应力和速度，并将获得的值与预定值进行比较; （e）确定剪切应力或速度是否大于或小于预定值，和（f）任选地修饰初始血管层并重复步骤（b） - （e）。 本发明还提供包含用于组织层的血管层的组合物以及具有血管层和试剂盒的医疗装置。
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公开(公告)号：EP3473093B1
公开(公告)日：2020-10-28
申请号：EP18211690.5
申请日：2008-04-14
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公开(公告)号：EP3473093A1
公开(公告)日：2019-04-24
申请号：EP18211690.5
申请日：2008-04-14
摘要： The invention provides an artificial vascular device comprising a plurality of stacked layers, wherein each stacked layer in the plurality of stacked layers comprises:
a vascular layer comprising a substrate defining a network of channels having at least one input channel, at least one output channel, and a plurality of intermediate channels connecting the at least one input channel and the at least one output channel, each channel having a height, a length, a width, and a diameter, wherein the intermediate channels are formed in accordance with Murray's law by varying said height and width with respect to adjacent portions of the channels,
a cell layer comprising a substrate defining a chamber for holding a plurality of cells, and
a membrane disposed interposing between the cell layer and the vascular layer.-
公开(公告)号：EP3192369A1
公开(公告)日：2017-07-19
申请号：EP16202467.3
申请日：2008-04-14
IPC分类号： A01N1/00
CPC分类号： A61F2/06 , A61F2/022 , A61F2/04 , A61F2/062 , A61F2002/068 , G01N33/5008 , G06F19/00
摘要： The invention provides method of fabricating a scaffold comprising a fluidic network, including the steps of: (a) generating an initial vascular layer for enclosing the chamber and providing fluid to the cells, the initial vascular layer having a network of channels for fluid; (b) translating the initial vascular layer into a model for fluid dynamics analysis; (c) analyzing the initial vascular layer based on desired parameters selected from the group consisting of a characteristic of a specific fluid, an input pressure, an output pressure, an overall flow rate and combinations thereof to determine sheer stress and velocity within the network of channels; (d) measuring the sheer stress and the velocity and comparing the obtained values to predetermined values; (e) determining if either of the shear stress or the velocity are greater than or less than the predetermined values, and (f) optionally modifying the initial vascular layer and repeating steps (b) - (e). The invention also provides compositions comprising a vascular layer for use in tissue lamina as well as a medical devices having a vascular layer and kits.
摘要翻译： 本发明提供了制造包含流体网络的支架的方法，包括以下步骤：（a）产生初始血管层以封闭腔室并向细胞提供流体，初始血管层具有用于流体的通道网络; （b）将初始血管层转化为流体动力学分析的模型; （c）基于选自由特定流体的特性，输入压力，输出压力，总流率及其组合组成的组中的期望参数分析初始血管层，以确定网络内的剪切应力和速度 渠道; （d）测量剪切应力和速度并将所得值与预定值进行比较; （e）确定剪切应力或速度中的任一个是大于还是小于预定值，以及（f）任选地修改初始脉管层并重复步骤（b） - （e）。 本发明还提供包含用于组织薄层的血管层的组合物以及具有血管层和试剂盒的医疗装置。
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公开(公告)号：EP2154955A2
公开(公告)日：2010-02-24
申请号：EP08742923.9
申请日：2008-04-14
IPC分类号： A01N1/00
CPC分类号： A61F2/06 , A61F2/022 , A61F2/04 , A61F2/062 , A61F2002/068 , G01N33/5008 , G06F19/00
摘要： The invention provides method of fabricating a scaffold comprising a fluidic network, including the steps of: (a) generating an initial vascular layer for enclosing the chamber and providing fluid to the cells, the initial vascular layer having a network of channels for fluid; (b) translating the initial vascular layer into a model for fluid dynamics analysis; (c) analyzing the initial vascular layer based on desired parameters selected from the group consisting of a characteristic of a specific fluid, an input pressure, an output pressure, an overall flow rate and combinations thereof to determine sheer stress and velocity within the network of channels; (d) measuring the sheer stress and the velocity and comparing the obtained values to predetermined values; (e) determining if either of the shear stress or the velocity are greater than or less than the predetermined values, and (f) optionally modifying the initial vascular layer and repeating steps (b) - (e). The invention also provides compositions comprising a vascular layer for use in tissue lamina as well as a medical devices having a vascular layer and kits.
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公开(公告)号：EP2254989A1
公开(公告)日：2010-12-01
申请号：EP09709997.2
申请日：2009-02-13
IPC分类号： C12N5/00
CPC分类号： G01N33/5014 , C12M25/04 , C12M35/08 , G01N33/5067
摘要： A well-based flow system for cell culture is described which provides for flow of culture containing compounds for drug screening to be exposed to cells seeded on a membrane. The flow of medium may be planar or radial and means are provided for the removal of waste media through fluid outlets in fluid communication with the assay well plates through conduits. Methods of using the system for cell culture and drug toxicity screening are also provided including coculturing cells such as hepatocytes, stem cells, fibroblasts and smooth muscle cells and selectively exposing cells to test compounds.
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