公开(公告)号：EP2315849B1

公开(公告)日：2017-11-08

申请号：EP09803662.7

申请日：2009-07-31

申请人： The Johns Hopkins University

发明人： DIEHL, Frank ,  DIAZ, Luis ,  KINZLER, Kenneth, W. ,  VOGELSTEIN, Bert ,  SCHMIDT, Kerstin

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2525/197 ,  C12Q2535/131 ,  C12Q2549/119 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/136

公开(公告)号：EP1948816B1

公开(公告)日：2011-12-07

申请号：EP06817236.0

申请日：2006-10-20

申请人： The Johns Hopkins University

发明人： VOGELSTEIN, Bert ,  DIEHL, Frank ,  KINZLER, Kenneth, W. ,  LI, Ming

IPC分类号： C12P19/34

CPC分类号： C12Q1/6858 ,  G01R31/025 ,  G01R31/3277 ,  C12Q2565/537 ,  C12Q2563/155 ,  C12Q2527/125

摘要： Improvements on the basic method used for BEAMing increase sensitivity and increase the signal-to-noise ratio. The improvements have permitted the determination of intrinsic error rates of various DNA polymerases and have permitted the detection of rare and subtle mutations in DNA isolated from plasma of cancer patients.

摘要翻译： 用于BEAMing的基本方法的改进提高了灵敏度并提高了信噪比。 这些改进允许确定各种DNA聚合酶的固有错误率，并允许检测从癌症患者血浆中分离的DNA中罕见且微妙的突变。

公开(公告)号：EP1948816A2

公开(公告)日：2008-07-30

申请号：EP06817236.0

申请日：2006-10-20

申请人： The Johns Hopkins University

发明人： VOGELSTEIN, Bert ,  DIEHL, Frank ,  KINZLER, Kenneth, W. ,  LI, Ming

IPC分类号： C12P19/34

CPC分类号： C12Q1/6858 ,  G01R31/025 ,  G01R31/3277 ,  C12Q2565/537 ,  C12Q2563/155 ,  C12Q2527/125

摘要： Improvements on the basic method used for BEAMing increase sensitivity and increase the signal-to-noise ratio. The improvements have permitted the determination of intrinsic error rates of various DNA polymerases and have permitted the detection of rare and subtle mutations in DNA isolated from plasma of cancer patients.

公开(公告)号：EP2315849A1

公开(公告)日：2011-05-04

申请号：EP09803662.7

申请日：2009-07-31

申请人： The Johns Hopkins University

发明人： DIEHL, Frank ,  DIAZ, Luis ,  KINZLER, Kenneth, W. ,  VOGELSTEIN, Bert ,  SCHMIDT, Kerstin

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2525/197 ,  C12Q2535/131 ,  C12Q2549/119 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/136

摘要： DNA containing somatic mutations is highly tumor specific and thus, in theory, can provide optimum markers. However, the number of circulating mutant gene fragments is small compared to the number of normal circulating DNA fragments, making it difficult to detect and quantify them with the sensitivity required for meaningful clinical use. We apply a highly sensitive approach to quantify circulating tumor DNA (ctDNA) in body samples of patients. Measurements of ctDNA can be used to reliably monitor tumor dynamics in subjects with cancer, especially those who are undergoing surgery or chemotherapy. This personalized genetic approach can be generally applied.

公开(公告)号：EP2038427A2

公开(公告)日：2009-03-25

申请号：EP07809665.8

申请日：2007-06-19

申请人： The Johns Hopkins University

发明人： DIEHL, Frank ,  KINZLER, Kenneth, W. ,  VOGELSTEIN, Bert

IPC分类号： C12Q1/68 ,  C07H21/02

CPC分类号： C12Q1/686 ,  C12Q1/6876 ,  C12Q2600/16 ,  C12Q2527/125 ,  C12Q2527/153 ,  C12Q2565/537

摘要： Modulation of the viscosity of the oil phase of a microemulsion used for amplification of DNA on a bead increases the homogeneity of product beads and the amount of amplified DNA per bead. Moreover the number of separate microemulsion populations that can be formed in parallel is increased using multi-well plates and mixer mill disruptor machines designed to lyse biological samples.