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    Modified green fluorescent proteins
    3.
    发明公开
    Modified green fluorescent proteins 失效
    Modifiziertegrün-fluoresineerende蛋白质

    公开(公告)号：EP1104769A2

    公开(公告)日：2001-06-06

    申请号：EP01105011.9

    申请日：1995-11-13

    申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    发明人： Tsien, Roger Y. ,  Heim, Roger

    IPC分类号： C07K14/435 ,  C12N5/10 ,  C12Q1/68 ,  C12N15/65

    CPC分类号： C07K14/43595 ,  C12N15/65 ,  G01N33/533 ,  G01N2333/43595 ,  Y10S530/855

    摘要： Modifications in the sequence of Aequorea wild-type GFP provide products having markedly different excitation and emission spectra from corresponding products from wild-type GFP. In one class of modifications, the product derived from the modified GFP exhibits an alteration in the ratio of two main excitation peaks observed with the product derived from wild-type GFP. In another class, the product derived from the modified GFP fluoresces at a shorter wavelength than the corresponding product from wild-type GFP. In yet another class of modifications, the product derived from the modified GFP exhibits only a single excitation peak and enhanced emission relative to the product derived from wild-type GFP.

    摘要翻译： 水稻野生型GFP序列中的修饰提供了来自野生型GFP的相应产物具有显着不同的激发和发射光谱的产物。 在一类修饰中，衍生自经修饰的GFP的产物表现出与用野生型GFP产生的产物观察到的两个主要激发峰的比率的变化。 在另一类中，衍生自经修饰的GFP的产物在比来自野生型GFP的相应产物更短的波长处发荧光。 在另一类修饰中，衍生自经修饰的GFP的产物相对于衍生自野生型GFP的产物仅显示单个激发峰和增强的发射。

    Scanning confocal microscope
    4.
    发明公开
    Scanning confocal microscope 失效
    Konfokales Rastermikroskop。

    公开(公告)号：EP0564178A1

    公开(公告)日：1993-10-06

    申请号：EP93302342.6

    申请日：1993-03-26

    申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    发明人： Tsien, Roger Y.

    IPC分类号： G02B21/00 ,  H04N1/04

    CPC分类号： H04N1/053 ,  G02B21/0076 ,  H04N2201/02443 ,  H04N2201/0471 ,  H04N2201/04734 ,  H04N2201/04746 ,  H04N2201/04768 ,  H04N2201/04798

    摘要： A scanning confocal microscopes scans a sample (16) with an incident beam of ultraviolet radiation, in a raster scan pattern, causing the sample to fluoresce and emit visible radiation, a portion of which retraces a portion of the path optical of incident beam, to a dichroic mirror (8) that separates it from the incident beam for detection by a photomultiplier (22,26) tube. A clock signal for clocking the output of the photomultiplier tube is provided by a reference beam system that directs a reference beam (RB) onto the same scanning mirror (9) as is the incident beam, at a coincident location on that mirror, and from there through a Ronchi grating (44) having a uniform series of alternating transparent and opaque regions. The resulting pulsed reference beam is detected by a second photomultiplier tube (46), to produce a clock signal that is an accurate representation of the instantaneous scan rate of the reference beam and, thereby, the scanning incident beam.

    摘要翻译： 扫描共聚焦显微镜以光栅扫描图案用入射紫外线束照射样品（16），导致样品发出荧光并发射其可见辐射（其一部分将入射光束的光路的一部分重新回溯到入射光束的一部分） 分离镜（8），其将入射光束与光电倍增管（22,26）管分离。 用于计时光电倍增管输出的时钟信号由参考光束系统提供，该参考光束系统将参考光束（RB）引导到与入射光束相同的扫描反射镜（9）上，在该反射镜上的重合位置 通过具有均匀系列的交替透明和不透明区域的Ronchi光栅（44）。 所产生的脉冲参考光束由第二光电倍增管（46）检测，以产生时钟信号，该时钟信号是参考光束的瞬时扫描速率，从而是扫描入射光束的精确表示。

    Modified green fluorescent proteins
    5.
    发明公开
    Modified green fluorescent proteins 失效
    改进的绿色荧光蛋白

    公开(公告)号：EP1104769A3

    公开(公告)日：2002-09-18

    申请号：EP01105011.9

    申请日：1995-11-13

    申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    发明人： Tsien, Roger Y. ,  Heim, Roger

    IPC分类号： C07K14/435 ,  C12N5/10 ,  C12Q1/68 ,  C12N15/65

    CPC分类号： C07K14/43595 ,  C12N15/65 ,  G01N33/533 ,  G01N2333/43595 ,  Y10S530/855

    摘要： Modifications in the sequence of Aequorea wild-type GFP provide products having different excitation and emission spectra from corresponding products from wild-type GFP. In one class of modifications, the product derived from the modified GFP exhibits an alteration in the ratio of two main excitation peaks observed with the product derived from wild-type GFP. In another class, the product derived from the modified GFP fluoresces at a shorter wavelength than the corresponding product from wild-type GFP. In yet another class of modifications, the product derived from the modified GFP exhibits only a single excitation peak and enhanced emission relative to the product derived from wild-type GFP.

    Scanning confocal microscope
    7.
    发明授权
    Scanning confocal microscope 失效
    共焦扫描显微镜

    公开(公告)号：EP0564178B1

    公开(公告)日：2000-07-12

    申请号：EP93302342.6

    申请日：1993-03-26

    申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    发明人： Tsien, Roger Y.

    IPC分类号： G02B21/00 ,  H04N1/04

    CPC分类号： H04N1/053 ,  G02B21/0076 ,  H04N2201/02443 ,  H04N2201/0471 ,  H04N2201/04734 ,  H04N2201/04746 ,  H04N2201/04768 ,  H04N2201/04798

    摘要： A scanning confocal microscopes scans a sample (16) with an incident beam of ultraviolet radiation, in a raster scan pattern, causing the sample to fluoresce and emit visible radiation, a portion of which retraces a portion of the path optical of incident beam, to a dichroic mirror (8) that separates it from the incident beam for detection by a photomultiplier (22,26) tube. A clock signal for clocking the output of the photomultiplier tube is provided by a reference beam system that directs a reference beam (RB) onto the same scanning mirror (9) as is the incident beam, at a coincident location on that mirror, and from there through a Ronchi grating (44) having a uniform series of alternating transparent and opaque regions. The resulting pulsed reference beam is detected by a second photomultiplier tube (46), to produce a clock signal that is an accurate representation of the instantaneous scan rate of the reference beam and, thereby, the scanning incident beam.

    Scanning confocal microscope providing a continuous display
    8.
    发明公开
    Scanning confocal microscope providing a continuous display 失效
    SCANNING CONFOCAL MICROSCOPE提供连续显示

    公开(公告)号：EP0592089A3

    公开(公告)日：1994-05-18

    申请号：EP93306533.6

    申请日：1993-08-18

    申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    发明人： Tsien, Roger Y.

    IPC分类号： G02B21/00 ,  G01N21/64 ,  H04N1/04 ,  G02B21/36

    CPC分类号： G02B21/008 ,  G02B21/0076

    摘要： A scanning confocal microscope scans a sample with an incident beam of radiation, in a raster scan pattern, causing the sample to fluoresce and emit visible radiation in at least two wavelengths. A portion of the fluorescent light retraces a portion of the path optical of incident beam, to a dichroic mirror that separates it from the incident beam for detection by a pair of photomultiplier tubes. A data processor accumulates digital data from the photomultiplier tubes to form a succession of image data frames of the sample being scanned, in the two wavelengths. Image data for a selected number of frames in each wavelength is averaged and then recorded on a single track of a recording system, in an alternating fashion with averaged data for the other wavelength. In addition, a ratio of the averaged data for the two wavelengths is delivered to a video display.