公开(公告)号：EP2139515B1

公开(公告)日：2018-06-13

申请号：EP08799746.6

申请日：2008-03-31

申请人： The Research Foundation of the State University of New York

发明人： WIMMER, Eckard ,  SKIENA, Steve ,  MUELLER, Steffen ,  FUTCHER, Bruce ,  PAPAMICHAIL, Dimitris ,  COLEMAN, John, Robert ,  CELLO, Jeronimo

IPC分类号： A61K39/13

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/13 ,  A61K39/145 ,  A61K2039/5254 ,  C07K14/005 ,  C12N2720/12361 ,  C12N2740/15061 ,  C12N2760/16061 ,  C12N2760/16121 ,  C12N2760/16134 ,  C12N2760/16162 ,  C12N2770/20061 ,  C12N2770/24161 ,  C12N2770/32061 ,  C12N2770/32621 ,  C12N2770/32634 ,  C12N2770/32661 ,  C12N2770/32662 ,  C12N2770/32671 ,  C12N2770/32721 ,  C12N2770/32761 ,  C12N2770/32762 ,  Y02A50/386 ,  Y02A50/388 ,  Y02A50/466

摘要： This invention provides an attenuated virus which comprises a modified viral genome containing nucleotide substitutions engineered in multiple locations in the genome, wherein the substitutions introduce synonymous deoptimized codons into the genome. The instant attenuated virus may be used in a vaccine composition for inducing a protective immune response in a subject. The invention also provides a method of synthesizing the instant attenuated virus. Further, this invention further provides a method for preventing a subject from becoming afflicted with a virus-associated disease comprising administering to the subject a prophylactically effective dose of a vaccine composition comprising the instant attenuated virus.

公开(公告)号：EP2139515A2

公开(公告)日：2010-01-06

申请号：EP08799746.6

申请日：2008-03-31

申请人： The Research Foundation of the State University of New York

发明人： WIMMER, Eckhard ,  SKIENA, Steve ,  MUELLER, Steffen ,  FUTCHER, Bruce ,  PAPAMICHAIL, Dimitris ,  COLEMAN, John, Robert ,  CELLO, Jeronimo

IPC分类号： A61K39/13

CPC分类号： C12N7/00 ,  A61K39/13 ,  A61K39/145 ,  A61K2039/5254 ,  C07K14/005 ,  C12N2720/12361 ,  C12N2740/15061 ,  C12N2760/16061 ,  C12N2760/16121 ,  C12N2760/16134 ,  C12N2760/16162 ,  C12N2770/20061 ,  C12N2770/24161 ,  C12N2770/32061 ,  C12N2770/32621 ,  C12N2770/32634 ,  C12N2770/32661 ,  C12N2770/32662 ,  C12N2770/32671 ,  C12N2770/32721 ,  C12N2770/32761 ,  C12N2770/32762 ,  Y02A50/386 ,  Y02A50/388 ,  Y02A50/466

摘要： This invention provides an attenuated virus which comprises a modified viral genome containing nucleotide substitutions engineered in multiple locations in the genome, wherein the substitutions introduce synonymous deoptimized codons into the genome. The instant attenuated virus may be used in a vaccine composition for inducing a protective immune response in a subject. The invention also provides a method of synthesizing the instant attenuated virus. Further, this invention further provides a method for preventing a subject from becoming afflicted with a virus-associated disease comprising administering to the subject a prophylactically effective dose of a vaccine composition comprising the instant attenuated virus.

摘要翻译： 本发明提供了一种减毒病毒，其包含在基因组中多个位置工程改造的含有核苷酸取代基的经修饰的病毒基因组，其中所述取代将同义脱酮密码子引入基因组。 本发明的减毒病毒可用于疫苗组合物中以在受试者中诱导保护性免疫应答。 本发明还提供了合成即时减毒病毒的方法。 此外，本发明进一步提供了一种防止受试者患有病毒相关疾病的方法，包括向受试者施用预防有效剂量的包含即时减毒病毒的疫苗组合物。