公开(公告)号：EP3341498A1

公开(公告)日：2018-07-04

申请号：EP16842702.9

申请日：2016-08-26

申请人： The Trustees of Columbia University in the City of New York

发明人： ALVAREZ, Mariano, Javier ,  CALIFANO, Andrea

IPC分类号： C12Q1/68 ,  G06F19/00 ,  G06F19/12

CPC分类号： G06F19/18 ,  G06F19/12 ,  G06F19/20

摘要： Methods for determining regulon enrichment in gene expression signatures are disclosed herein. An example method can include obtaining a set of transcriptional targets of a regulon. The method can include obtaining a gene expression signature by comparing a gene expression profile of a test sample to gene expression profiles of a plurality of samples representing control phenotypes. The method can include calculating a regulon enrichment score for each regulon in the gene expression signature. The method can including determining whether a number of control samples in the control phenotypes is above a predetermined threshold to support evaluation of statistical significance using permutation analysis. The method can include, in response to determining that the number of control samples is above the predetermined threshold, calculating a significance value by comparing each regulon enrichment score to a null model.

公开(公告)号：EP3340996A1

公开(公告)日：2018-07-04

申请号：EP16842698.9

申请日：2016-08-26

申请人： The Trustees of Columbia University in the City of New York

发明人： CALIFANO, Andrea ,  ALVAREZ, Mariano, Javier

IPC分类号： A61K35/13 ,  C12N5/09 ,  G01N33/574

CPC分类号： G06F19/12 ,  G01N33/57484 ,  G01N33/68 ,  G01N2500/20

摘要： Techniques to profile a disease or a disorder (e.g., a tumor) based on a protein activity signature are disclosed herein. An example method can include measuring quantitatively protein activity of a plurality of master regulator proteins in a sample from a disease or disorder; and profiling the tumor from the quantitative protein activity of the master regulator proteins. Also disclosed are methods of identifying a compound or compounds that treats diseases or disorders (e.g., inhibit tumor cell growth).