公开(公告)号：EP2139514A1

公开(公告)日：2010-01-06

申请号：EP07754057.3

申请日：2007-03-27

申请人： The United States of America, as represented by the Secretary of the Army, Walter Reed Army Institute of Research

发明人： OAKS, Edwin V. ,  TURBYFILL, Kevin R. ,  KAMINSKI, Robert W.

IPC分类号： A61K39/02 ,  A61P31/04 ,  A61K39/112 ,  A61K39/108

CPC分类号： A61K39/0283 ,  A61K39/39 ,  A61K47/646 ,  A61K2039/543 ,  A61K2039/55516 ,  A61K2039/55572 ,  A61K2039/64 ,  Y02A50/474 ,  Y02A50/476 ,  Y02A50/482

摘要： An artificial invasin complex is prepared from purified or recombinantly prepared invasins and gram negative bacteria lipopolysaccharides. Typically, IpaB is mixed with IpaC to form a IpaB:IpaC complex. This invasin protein complex is then mixed with the lipopolysaccharide to form an artificial invasin complex. Additional bioactive molecules can be incorporated into the complex during manufacture. This artificial invasin complex is similar in function to native Invaplex 24 or Invaplex 50. The artificial invasin complex has superior immunogenicity properties relative to the native complex and can be taylor made. Its method of preparation lends itself to scale up. The artificial invasin complex can facilitate transport of biomolecules, therapeutics and antibiotics across cell membranes in a manner similar to native Shigella Invaplex.

摘要翻译： 从纯化或重组制备的侵染素和革兰氏阴性细菌脂多糖制备人造侵染素复合物。 通常，IpaB与IpaC混合形成IpaB：IpaC复合体。 然后将这种侵入蛋白复合物与脂多糖混合形成人造侵袭素复合物。 额外的生物活性分子可以在制造期间结合到复合物中。 这种人造侵染素复合物在功能上与天然Invaplex 24或Invaplex 50类似。人造侵染素复合物相对于天然复合物具有优异的免疫原性，并且可以是泰勒制造的。 其制备方法有助于扩大规模。 人造侵染素复合物可以促进生物分子，治疗剂和抗生素以类似于天然志贺氏菌Invaplex的方式跨细胞膜转运。

公开(公告)号：EP2139514B1

公开(公告)日：2014-05-21

申请号：EP07754057.3

申请日：2007-03-27

申请人： The United States of America, as represented by The Secretary of the Army, Walter Reed Army Institute of Research

发明人： OAKS, Edwin V. ,  TURBYFILL, Kevin R. ,  KAMINSKI, Robert W.

IPC分类号： A61K39/02 ,  A61P31/04 ,  A61K39/112 ,  A61K39/108

CPC分类号： A61K39/0283 ,  A61K39/39 ,  A61K47/646 ,  A61K2039/543 ,  A61K2039/55516 ,  A61K2039/55572 ,  A61K2039/64 ,  Y02A50/474 ,  Y02A50/476 ,  Y02A50/482

摘要： An artificial invasin complex is prepared from purified or recombinantly prepared invasins and gram negative bacteria lipopolysaccharides. Typically, IpaB is mixed with IpaC to form a IpaB:IpaC complex. This invasin protein complex is then mixed with the lipopolysaccharide to form an artificial invasin complex. Additional bioactive molecules can be incorporated into the complex during manufacture. This artificial invasin complex is similar in function to native Invaplex 24 or Invaplex 50. The artificial invasin complex has superior immunogenicity properties relative to the native complex and can be taylor made. Its method of preparation lends itself to scale up. The artificial invasin complex can facilitate transport of biomolecules, therapeutics and antibiotics across cell membranes in a manner similar to native Shigella Invaplex.