公开(公告)号：EP3865212A1

公开(公告)日：2021-08-18

申请号：EP21151023.5

申请日：2014-03-28

申请人： The University of British Columbia

发明人： RICICOVA, Marketa ,  HEYRIES, Kevin Albert ,  ZAHN, Hans ,  PETRIV, Oleh ,  LECAULT, Veronique ,  SINGHAL, Anupam ,  DA COSTA, Daniel J ,  HANSEN, Carl Lars Genghis ,  NELSON, Brad ,  NIELSEN, Julie ,  LISAINGO, Kathleen

IPC分类号： B01L3/00 ,  C07K16/00 ,  C07K16/10 ,  C07K16/40 ,  G01N33/50 ,  G01N33/543

摘要： Methods and devices are provided herein for identifying a cell population comprising an effector cell that exerts an extracellular effect. In one embodiment the method comprises retaining in a microreactor a cell population comprising one or more effector cells, wherein the contents of the microreactor further comprise a readout particle population comprising one or more readout particles, incubating the cell population and the readout particle population within the microreactor, assaying the cell population for the presence of the extracellular effect, wherein the readout particle population or subpopulation thereof provides a direct or indirect readout of the extracellular effect, and determining, based on the results of the assaying step, whether one or more effector cells within the cell population exerts the extracellular effect on the readout particle. If an extracellular effect is measured, the cell population is recovered for further analysis to determine the cell or cells responsible for the effect.