公开(公告)号：EP3394326A1

公开(公告)日：2018-10-31

申请号：EP16877036.0

申请日：2016-12-21

申请人： The University of British Columbia

发明人： FALCONER, Ester ,  HANSEN, Carl Lars Genghis ,  LECAULT, Veronique ,  LISAINGO, Kathleen ,  HEYRIES, Kevin Albert

IPC分类号： C40B30/04 ,  C12N5/078 ,  C12Q1/02 ,  C12Q1/68 ,  C40B20/00 ,  C40B40/02 ,  C40B50/06 ,  G01N33/543 ,  G01N33/566 ,  G01N33/574 ,  G01N33/68

CPC分类号： C40B40/02 ,  C12N15/1034 ,  C12N15/1037 ,  C12Q1/68 ,  C40B50/06 ,  G01N33/566 ,  G01N33/6845 ,  G01N33/6854

摘要： The present invention relates to methods for isolating the sequences of an antibody that reacts with a disease related antigen, e.g., an autoantigen, without knowing the identity of the antigen (sequence or structural epitope) a priori. The methods can also be used to identify an antigen that mediates a disease state, e.g., an autoantigen implicated in an autoimmune disorder or a tumor response.

公开(公告)号：EP2714884B1

公开(公告)日：2019-08-21

申请号：EP11867040.5

申请日：2011-05-27

申请人： The University of British Columbia

发明人： HANSEN, Carl Lars Genghis ,  VANINSBERGHE, Michael ,  WHITE, Adam ,  PETRIV, Oleh ,  LEAVER, Tim ,  SINGHAL, Anupam ,  BOWDEN, William ,  LECAULT, Veronique ,  DA COSTA, Dan ,  WU, Leo ,  RUSSELL, Georgia ,  SIKORSKI, Darek

IPC分类号： B01L3/00 ,  C12M3/06 ,  G01N33/50 ,  G01N33/543 ,  G01N33/569 ,  C12Q1/6844 ,  B01L7/00

公开(公告)号：EP2978855A1

公开(公告)日：2016-02-03

申请号：EP14775104.4

申请日：2014-03-28

申请人： The University of British Columbia

发明人： RICICOVA, Marketa ,  HEYRIES, Kevin Albert ,  ZAHN, Hans ,  PETRIV, Oleh ,  LECAULT, Veronique ,  SINGHAL, Anupam ,  DA COSTA, Daniel, J. ,  HANSEN, Carl, L., G. ,  NELSON, Brad ,  NIELSEN, Julie ,  LISAINGO, Kathleen

IPC分类号： C12Q1/02 ,  C12M1/34 ,  C12Q1/68 ,  G01N33/48 ,  G01N33/567

CPC分类号： G01N33/5052 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/12 ,  B01L2300/04 ,  B01L2300/0627 ,  B01L2300/0838 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/14 ,  C07K16/00 ,  C07K16/1018 ,  C07K16/40 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/70 ,  C12N5/0634 ,  C12N5/0635 ,  C12Q1/6888 ,  G01N33/5047 ,  G01N33/505 ,  G01N33/54313 ,  G01N33/6845 ,  G01N33/6854 ,  G01N35/0099

摘要： Methods and devices are provided herein for identifying a cell population comprising an effector cell that exerts an extracellular effect. In one embodiment the method comprises retaining in a microreactor a cell population comprising one or more effector cells, wherein the contents of the microreactor further comprise a readout particle population comprising one or more readout particles, incubating the cell population and the readout particle population within the microreactor, assaying the cell population for the presence of the extracellular effect, wherein the readout particle population or subpopulation thereof provides a direct or indirect readout of the extracellular effect, and determining, based on the results of the assaying step, whether one or more effector cells within the cell population exerts the extracellular effect on the readout particle. If an extracellular effect is measured, the cell population is recovered for further analysis to determine the cell or cells responsible for the effect.

摘要翻译： 本文提供了用于鉴定包含发挥细胞外作用的效应细胞的细胞群的方法和装置。 在一个实施方案中，所述方法包括将包含一种或多种效应细胞的细胞群保留在微反应器中，其中所述微反应器的内容物还包含含有一种或多种读出颗粒的读出颗粒群，将细胞群和读出颗粒群在 测定细胞群存在细胞外效应，其中读出颗粒群或其亚群提供细胞外效应的直接或间接读数，并基于测定步骤的结果确定一种或多种效应物 细胞群体内的细胞对读出颗粒施加细胞外作用。 如果测量胞外作用，则将细胞群回收用于进一步分析以确定负责该作用的细胞或细胞。

公开(公告)号：EP3865212A1

公开(公告)日：2021-08-18

申请号：EP21151023.5

申请日：2014-03-28

申请人： The University of British Columbia

发明人： RICICOVA, Marketa ,  HEYRIES, Kevin Albert ,  ZAHN, Hans ,  PETRIV, Oleh ,  LECAULT, Veronique ,  SINGHAL, Anupam ,  DA COSTA, Daniel J ,  HANSEN, Carl Lars Genghis ,  NELSON, Brad ,  NIELSEN, Julie ,  LISAINGO, Kathleen

IPC分类号： B01L3/00 ,  C07K16/00 ,  C07K16/10 ,  C07K16/40 ,  G01N33/50 ,  G01N33/543

摘要： Methods and devices are provided herein for identifying a cell population comprising an effector cell that exerts an extracellular effect. In one embodiment the method comprises retaining in a microreactor a cell population comprising one or more effector cells, wherein the contents of the microreactor further comprise a readout particle population comprising one or more readout particles, incubating the cell population and the readout particle population within the microreactor, assaying the cell population for the presence of the extracellular effect, wherein the readout particle population or subpopulation thereof provides a direct or indirect readout of the extracellular effect, and determining, based on the results of the assaying step, whether one or more effector cells within the cell population exerts the extracellular effect on the readout particle. If an extracellular effect is measured, the cell population is recovered for further analysis to determine the cell or cells responsible for the effect.

公开(公告)号：EP4190951A1

公开(公告)日：2023-06-07

申请号：EP22194954.8

申请日：2016-12-21

申请人： The University of British Columbia

发明人： FALCONER, Ester ,  HANSEN, Carl Lars Genghis ,  LECAULT, Veronique ,  LISAINGO, Kathleen ,  HEYRIES, Kevin Albert

IPC分类号： C40B30/04 ,  C12N5/078 ,  C12Q1/02 ,  C12Q1/68 ,  C40B20/00 ,  C40B40/02 ,  C40B50/06 ,  G01N33/543 ,  G01N33/566 ,  G01N33/574 ,  G01N33/68

摘要： The present invention relates to methods for isolating the sequences of an antibody that reacts with a disease related antigen, e.g., an autoantigen, without knowing the identity of the antigen (sequence or structural epitope) a priori. The methods can also be used to identify an antigen that mediates a disease state, e.g., an autoantigen implicated in an autoimmune disorder or a tumor response.

公开(公告)号：EP2714884A1

公开(公告)日：2014-04-09

申请号：EP11867040.5

申请日：2011-05-27

申请人： The University of British Columbia

发明人： HANSEN, Carl Lars Genghis ,  VANINSBERGHE, Michael ,  WHITE, Adam ,  PETRIV, Oleh ,  LEAVER, Tim ,  SINGHAL, Anupam ,  BOWDEN, William ,  LECAULT, Veronique ,  DA COSTA, Dan ,  WU, Leo ,  RUSSELL, Georgia ,  SIKORSKI, Darek

IPC分类号： C12M1/00 ,  C12M3/00 ,  C12N1/00 ,  C12N5/07 ,  C12Q1/02 ,  C12Q1/68 ,  G01N1/28 ,  G01N33/50

CPC分类号： C12Q1/6806 ,  B01L3/502753 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2400/086 ,  C12M23/16 ,  C12Q1/6844 ,  G01N1/34 ,  G01N33/5005 ,  G01N33/54366 ,  G01N33/569

摘要： Microfluidic devices are provided for trapping, isolating, and processing single cells. The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. The devices may further include auxiliary chambers integrated with the cell capture chamber for subsequent processing and assaying of the contents of a captured cell. Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber.