公开(公告)号：EP1105736A1

公开(公告)日：2001-06-13

申请号：EP99942403.9

申请日：1999-08-24

申请人： Therasense, Inc.

发明人： HELLER, Adam ,  CAMPBELL, Charles, N.

IPC分类号： G01N33/543 ,  G01N27/327 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/28

CPC分类号： G01N33/543 ,  C12Q1/002 ,  C12Q1/005 ,  G01N33/5438 ,  Y10S435/817 ,  Y10S436/806

摘要： An electrochemical affinity assay system for detection of ligand-ligand receptor binding. The first member of a ligand-ligand receptor pair is immobilized in a redox polymer. The second member is bound then to the first. The third member is labeled with the detection marker horseradish peroxidase or soybean peroxidase. Binding of the labeled third member to the electrode via the second member results in electrical contact between the peroxidase and the redox polymer, causing the electrical connection of the reaction centers of the peroxidase label to the electrode through the conducting redox polymer. Such connection converts the film to a catalyst for the electroreduction of the hydrogen peroxide produced within the film by the immobilized substrate-generating enzyme.

公开(公告)号：EP1105736B1

公开(公告)日：2002-06-05

申请号：EP99942403.9

申请日：1999-08-24

申请人： Therasense, Inc.

发明人： HELLER, Adam ,  CAMPBELL, Charles, N.

IPC分类号： G01N33/543 ,  G01N27/327 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/28

CPC分类号： G01N33/543 ,  C12Q1/002 ,  C12Q1/005 ,  G01N33/5438 ,  Y10S435/817 ,  Y10S436/806

摘要： An electrochemical affinity assay system for detection of ligand-ligand receptor binding. The first member of a ligand-ligand receptor pair is immobilized in a redox polymer. The second member is bound then to the first. The third member is labeled with the detection marker horseradish peroxidase or soybean peroxidase. Binding of the labeled third member to the electrode via the second member results in electrical contact between the peroxidase and the redox polymer, causing the electrical connection of the reaction centers of the peroxidase label to the electrode through the conducting redox polymer. Such connection converts the film to a catalyst for the electroreduction of the hydrogen peroxide produced within the film by the immobilized substrate-generating enzyme.