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    DNA POLYMERASES
    2.
    发明授权
    DNA POLYMERASES 有权

    公开(公告)号：EP3026111B1

    公开(公告)日：2018-06-06

    申请号：EP15185052.6

    申请日：2012-08-02

    Applicant: Thermo Fisher Scientific Baltics UAB

    Inventor： SKIRGAILA, Remigijus ,  TUBELEVICIUTE, Agne ,  RIMSELIENE, Renata ,  BURINSKAS, Sigitas

    IPC: C12N9/12

    CPC classification number: C12N9/1252 ,  C07K2319/21 ,  C12Y207/07007

    Abstract: A DNA polymerase mutant comprising a Taq DNA polymerase amino acid sequence with a mutation at one or more of the following selected amino acid positions: E189K, E230K, E507K, H28R, L30R, G38R, F73V, H75R, E76A, E76G, E76K, E90K, K206R, E315K, A348V, L351F, A439T, D452N, G504S, E507A, D551N, L552R, 1553V, D578N, H676R, Q680R, D732G, E734G, E734K, F749V; wherein the polymerase mutant exhibits relative to wild-type DNA polymerase increased polymerase speed, increased affinity to DNA substrate and/or increased resistance to a DNA polymerase inhibitor; and wherein, when the mutation is E507K in combination with two or more further mutations or the mutation is Q680R in combination with four or more further mutations, at least one of the further mutations is at one of the selected amino acid positions; and when the mutation is 1553V, this is not in combination with D551S.

    DNA POLYMERASES
    5.
    发明公开
    DNA POLYMERASES 有权
    DNA-POLYMERASEN

    公开(公告)号：EP3026111A3

    公开(公告)日：2016-08-31

    申请号：EP15185052.6

    申请日：2012-08-02

    Applicant: Thermo Fisher Scientific Baltics UAB

    Inventor： SKIRGAILA, Remigijus ,  TUBELEVICIUTE, Agne ,  RIMSELIENE, Renata ,  BURINSKAS, Sigitas

    IPC: C12N9/12

    CPC classification number: C12N9/1252 ,  C07K2319/21 ,  C12Y207/07007

    Abstract: A DNA polymerase mutant comprising SEQ ID NO:13 with a mutation at position E230 and/or E507 and optionally one or more of the following mutations E189K, H28R, L30R, L30P, G38R, F73V, H75R, E76G, E76K, E76A, E90K, K206R, E315K, A348V, L351 F, A439T, D452N, G504S, G504D, D551N, L552R, 1553V, D578N, H676R, Q680R, D732G, E734G, E734K, F749V;
    wherein the polymerase mutant exhibits relative to wild-type DNA polymerase of SEQ ID NO: 13, and under the same conditions, increased polymerase speed and at least one of increased affinity to DNA substrate and/or increased resistance to a DNA polymerase inhibitor.

    Abstract translation: 一种DNA聚合酶突变体，其包含在一个或多个以下选择的氨基酸位置具有突变的Taq DNA聚合酶氨基酸序列：E189K，E230K，E507K，H28R，L30R，G38R，F73V，H75R，E76A，E76G，E76K，E90K ，K206R，E315K，A348V，L351F，A439T，D452N，G504S，E507A，D551N，L552R，1553V，D578N，H676R，Q680R，D732G，E734G，E734K，F749V; 其中所述聚合酶突变体相对于野生型DNA聚合酶显示出增加的聚合酶速度，对DNA底物的增加的亲和力和/或对DNA聚合酶抑制剂的增加的抗性; 并且其中当所述突变与两个或更多个其它突变组合时为E507K时，或突变为Q680R与四个或更多个其它突变组合时，至少一个其它突变位于选定氨基酸位置之一; 当突变为1553V时，不与D551S组合。

    DNA POLYMERASES
    6.
    发明公开
    DNA POLYMERASES 有权
    DNA聚合物

    公开(公告)号：EP3026111A2

    公开(公告)日：2016-06-01

    申请号：EP15185052.6

    申请日：2012-08-02

    Applicant: Thermo Fisher Scientific Baltics UAB

    Inventor： SKIRGAILA, Remigijus ,  TUBELEVICIUTE, Agne ,  RIMSELIENE, Renata ,  BURINSKAS, Sigitas

    IPC: C12N9/12

    CPC classification number: C12N9/1252 ,  C07K2319/21 ,  C12Y207/07007

    Abstract: A DNA polymerase mutant comprising SEQ ID NO:13 with a mutation at position E230 and/or E507 and optionally one or more of the following mutations E189K, H28R, L30R, L30P, G38R, F73V, H75R, E76G, E76K, E76A, E90K, K206R, E315K, A348V, L351 F, A439T, D452N, G504S, G504D, D551N, L552R, 1553V, D578N, H676R, Q680R, D732G, E734G, E734K, F749V;
    wherein the polymerase mutant exhibits relative to wild-type DNA polymerase of SEQ ID NO: 13, and under the same conditions, increased polymerase speed and at least one of increased affinity to DNA substrate and/or increased resistance to a DNA polymerase inhibitor.

    Abstract translation: 包含SEQ ID NO：13的DNA聚合酶突变体，其在位置E230和/或E507具有突变，并且任选地存在一个或多个以下突变E189K，H28R，L30R，L30P，G38R，F73V，H75R，E76G，E76K，E76A，E90K ，K206R，E315K，A348V，L351F，A439T，D452N，G504S，G504D，D551N，L552R，1553V，D578N，H676R，Q680R，D732G，E734G，E734K，F749V; 其中所述聚合酶突变体相对于SEQ ID NO：13的野生型DNA聚合酶展现出并且在相同条件下增加了聚合酶速度和对DNA底物增加的亲和力和/或增加的对DNA聚合酶抑制剂的抗性中的至少一种。

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