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    METHOD FOR SEPARATING CELL FROM BIOLOGICAL TISSUE
    1.
    发明公开
    METHOD FOR SEPARATING CELL FROM BIOLOGICAL TISSUE 审中-公开
    VERFAHREN ZUMABLÖSENVON ZELLEN VON EINEM生物学家GEWEBE

    公开(公告)号：EP2952577A1

    公开(公告)日：2015-12-09

    申请号：EP14745765.9

    申请日：2014-01-31

    申请人： Tohoku University ,  Meiji Seika Pharma Co., Ltd. ,  National University Corporation Tokyo University of Agriculture and Technology

    发明人： GOTO, Masafumi ,  MURAYAMA, Kazutaka ,  YAMAGATA, Youhei ,  WATANABE, Kimiko

    IPC分类号： C12N5/071 ,  C12N1/00 ,  C12N5/07

    CPC分类号： C12N5/0676 ,  C12N5/00 ,  C12N2509/00

    摘要： Provided is a method for efficiently and stably separating cells having a high biological activity from a biological tissue, by using a degrading-enzyme composition, which is prepared by adding an enzyme for degrading a major protein of the biological tissue in an amount determined depending on the composition of the major protein to a predetermined amount of a neutral protease and/or a protease derived from Clostridium sp. According to this method, the type and amount of protein-degrading enzyme to be used for isolating cells can be determined from the composition of a major protein of the biological tissue. Thus, cells having a high biological activity can be efficiently separated while reducing the amount of protein-degrading enzyme to be used.

    摘要翻译： 提供了通过使用降解酶组合物来有效且稳定地分离具有高生物活性的细胞的方法，所述降解酶组合物通过添加用于降解生物组织的主要蛋白质的酶，其量取决于 主要蛋白质与预定量的中性蛋白酶和/或衍生自梭菌属的蛋白酶的组成。 根据该方法，可以从生物组织的主要蛋白质的组成来确定用于分离细胞的蛋白质降解酶的类型和量。 因此，可以有效地分离具有高生物活性的细胞，同时减少使用的蛋白质降解酶的量。