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1.发明公开METHODS, KITS AND REACTION MIXTURES FOR ANALYZING SINGLE-STRANDED NUCLEIC ACID SEQUENCES 有权方法，试剂盒和反应混合物，例如核酸分析序列的单
公开(公告)号：EP2491144A4
公开(公告)日：2013-08-07
申请号：EP10825677
申请日：2010-10-21
申请人： UNIV BRANDEIS
发明人： WANGH LAWRENCE , RICE JOHN , REIS ARTHUR , SANCHEZ J AQUILES
CPC分类号： C12Q1/689 , C12Q1/6818 , C12Q1/6827 , C12Q1/6858 , C12Q1/6886 , C12Q2600/156 , C12Q2565/1015 , C12Q2537/101 , C12Q2527/107 , C12Q2531/101 , C12Q2531/107 , C12Q2537/143 , C12Q2565/101
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公开(公告)号：EP2948564A4
公开(公告)日：2016-10-05
申请号：EP14743084
申请日：2014-01-23
申请人： UNIV BRANDEIS
发明人： RICE JOHN , WANGH LAWRENCE J , REIS JR ARTHUR H , PIERCE KENNETH , HARTSHORN CRISTINA , SANCHEZ J AQUILES , VAN HOOSER STEPHEN , FISHBEIN SKYE
CPC分类号： C12Q1/701 , C12Q1/6848 , C12Q2525/186 , C12Q2527/107 , C12Q2527/125 , C12Q2527/143 , C12Q2565/101
摘要： Provided herein are reagents for improving PCR accuracy.
摘要翻译： 提供了用于PCR提高精确度的试剂。
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公开(公告)号：EP1210358A4
公开(公告)日：2005-01-05
申请号：EP00955479
申请日：2000-08-14
申请人： UNIV BRANDEIS
CPC分类号： C12Q1/6876 , C12Q1/6806 , C12Q1/686 , C12Q2600/16 , C12Q2521/537 , C12Q2565/1015 , C12Q2547/107
摘要： Disclosed are compositions, methods, and kits useful for detection of the presence and/or quantity of one or more chromosomes from single cells, groups of cells, or subcellular compartments. Provided is a lysis buffer for the preparation of substantially accessible nucleic acid molecules from a single cell. Also provided are moderately-repeated highly-conserved nucleic acid sequences, and oligonucleotide primer and probe molecules which hybridize specifically thereto. Methods for the detection of the presence or quantity of one or more chromosomes from a single cell are included, as are methods for the assessment of the reliability of the results of the methods of the invention. Kits for the convenient practice of the invention are also included.
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4.发明公开DETECTION OF SEQUENCE VARIANTS IN THE HUMAN EPIDERMAL GROWTH FACTOR RECEPTOR (EGFR) GENE 审中-公开表皮生长因子受体（EGFR）基因序列变异的证据
公开(公告)号：EP2715347A4
公开(公告)日：2014-12-03
申请号：EP12790203
申请日：2012-05-25
申请人： UNIV BRANDEIS
CPC分类号： C12Q1/6827 , C12Q1/6818 , C12Q1/686 , C12Q1/6886 , C12Q2600/156 , C12Q2527/107 , C12Q2531/107 , C12Q2565/1015
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公开(公告)号：EP1468114A4
公开(公告)日：2005-05-25
申请号：EP02795963
申请日：2002-12-19
申请人： UNIV BRANDEIS
IPC分类号： G01N33/53 , C07H21/00 , C12N15/09 , C12Q1/68 , G01N1/28 , G01N33/566 , C07H19/00 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/686 , C07H21/00 , C12Q1/6806 , C12Q1/6851 , C12Q2561/113 , C12Q2531/107 , C12Q2527/107
摘要： A non-symmetric polymerase chain reaction (PCR) amplification method employing a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25°C below the melting temperature of the amplicon. Assays employing such amplification and labeled hybridization probes, including assays that include a detection step following primer extension or a low-temperature probe, or both. Kits for performing such assays and primer or primerand-probe sets for performing the foregoing amplifications and assays.
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