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    NUCLEIC ACID SEQUENCING BY NANOPORE DETECTION OF TAG MOLECULES
    1.
    发明公开
    NUCLEIC ACID SEQUENCING BY NANOPORE DETECTION OF TAG MOLECULES 审中-公开
    NUCLEINSÄURESEQUENZIERUNGDURCH NANOPORENDETEKTION VONMARKIERUNGSMOLEKÜLEN

    公开(公告)号：EP2864502A4

    公开(公告)日：2016-04-13

    申请号：EP13807639

    申请日：2013-04-08

    申请人： UNIV COLUMBIA ,  GENIA TECHNOLOGIES INC

    发明人： JU JINGYUE ,  DAVIS RANDALL ,  CHEN ROGER

    IPC分类号： C12Q1/68 ,  C12Q1/00

    CPC分类号： C12Q1/6869 ,  G01N33/48721 ,  C12Q2521/101 ,  C12Q2521/543 ,  C12Q2537/157 ,  C12Q2565/631

    摘要： This disclosure provides systems and methods for sequencing nucleic acids using nucleotide analogues and translocation of tags from incorporated nucleotide analogues through a nanopore. In aspects, this disclosure is related to composition, method, and system for sequencing a nucleic acid using tag molecules and detection of translocation through a nanopore of tags released from incorporation of the molecule.

    摘要翻译： 本公开提供了使用核苷酸类似物对核酸进行测序的系统和方法，以及通过纳米孔从掺入的核苷酸类似物转移标签。 在一些方面，本公开涉及使用标签分子对核酸测序的组合物，方法和系统，以及通过从分子掺入释放的标记的纳米孔的易位检测。

    NUCLEIC ACID SEQUENCING USING TAGS
    3.
    发明公开
    NUCLEIC ACID SEQUENCING USING TAGS 审中-公开
    NUKLEINSÄURESEQUENZIERUNGUNTER VERWENDUNG VON标签

    公开(公告)号：EP2917372A4

    公开(公告)日：2016-10-05

    申请号：EP13853114

    申请日：2013-11-07

    申请人： GENIA TECHNOLOGIES INC

    发明人： DAVIS RANDALL W ,  CHEN ROGER ,  BIBILLO ARKADIUSZ ,  KORENBLUM DANIEL ,  DORWART MICHAEL

    IPC分类号： C12Q1/68 ,  G01N33/48

    CPC分类号： C12Q1/6869 ,  G01N27/44717 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2527/113 ,  C12Q2535/122 ,  C12Q2563/113 ,  C12Q2565/631

    摘要： This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.

    摘要翻译： 本公开提供了用于对核酸样品进行测序的芯片，系统和方法。 将标记的核苷酸提供到包含膜中的纳米孔的反应室中。 标记的核苷酸的单个标记的核苷酸可以含有与核苷酸偶联的标签，该标签借助于纳米孔可检测。 接下来，标记的核苷酸的单个标记的核苷酸可以掺入与衍生自核酸样品的单链核酸分子互补的生长链中。 借助于纳米孔，可以在掺入个体标记的核苷酸时检测与单个标记的核苷酸相关的标签。 当标签从核苷酸释放时，可以借助纳米孔检测标签。

    NANOPORE BASED MOLECULAR DETECTION AND SEQUENCING
    5.
    发明公开
    NANOPORE BASED MOLECULAR DETECTION AND SEQUENCING 审中-公开
    鉴定及测序分子对纳米多孔质基体

    公开(公告)号：EP2807476A4

    公开(公告)日：2015-12-09

    申请号：EP13738391

    申请日：2013-01-18

    申请人： GENIA TECHNOLOGIES INC

    发明人： DAVIS RANDALL ,  CHEN ROGER

    IPC分类号： G01N27/26 ,  C12Q1/68 ,  G01N27/414 ,  G01N33/487

    CPC分类号： C12Q1/6869 ,  C12Q1/6874 ,  G01N33/48721 ,  C12Q2521/101 ,  C12Q2565/607

    摘要： This disclosure provides systems and methods for molecular identification and polymer (e.g., nucleic acid) sequencing using nanopores. The polymer may be passed through or in proximity to the nanopore and various subunits of the polymer may affect the current flowing through the nanopore. The various subunits may be identified by measuring the current at a plurality of voltages applied across the nanopore and/or membrane. In some cases, the polymerization of tagged nucleotides presents tag molecules to the nanopore that can be identified by measuring the current at a plurality of voltages applied across the nanopore and/or membrane. Also provided herein are systems and methods for sequencing both the sense and anti-sense strand of a double stranded nucleic acid molecule with a nanopore and methods for using ribonucleic acid (RNA) speed bump molecules to slow the passage of a nucleic acid molecule through or in proximity to a nanopore.