公开(公告)号：EP2820141A4

公开(公告)日：2016-01-27

申请号：EP13755329

申请日：2013-02-27

申请人： UNIV DUKE

发明人： REITMAN ZACHARY JAMES ,  YAN HAI ,  CHOI BRYAN DAEHAHN ,  SAMPSON JOHN HOWARD

IPC分类号： C12N9/04 ,  C12N1/00 ,  C12N1/20 ,  C12P7/00 ,  C12P7/40

CPC分类号： C12N15/52 ,  C07K2319/21 ,  C12N9/0006 ,  C12N9/0008

摘要： Described herein are compositions and methods for generating oxidoreductases for enantioselective reactions. Described herein are compositions and methods for generating neomorphic (R)-2-hydroxyacid dehydrogenases capable of enzymatically converting a 1-carboxy-2-ketoacid to a 1-carboxy-(R)-2-hydroxyacid, or the reverse reaction. Illustrative examples include (a) (R)-2-hydroxyadipate dehydrogenase and uses thereof for converting 2-oxoadipate to (R)-2-hydroxyadipate, or the reverse reaction; and (b) (R)-2-hydroxyglutarate dehydrogenase and uses thereof for converting 2-oxoglutarate to (R)-2-hydroxyglutarate, or the reverse reaction. Also described herein are compositions and methods for generating non-natural microbial organisms to enzymatically convert 2-oxoadipate to (E)-2-hexenedioate or adipate, or to enzymatically convert 2-oxoglutarate to (E)-2-pentenedioate or glutarate, or the respective reverse reactions.

摘要翻译： 本文描述了用于产生对映选择性反应的氧化还原酶的组合物和方法。 本文描述的是用于产生能够将1-羧基-2-酮酸酸化为1-羧基 - （R）-2-羟基酸或逆反应的新型（R）-2-羟基酸脱氢酶的组合物和方法。 示例性实例包括（a）（R）-2-羟基己二酸脱氢酶及其用于将2-氧代己二酸转化为（R）-2-羟基己二酸酯的用途或反向反应; 和（b）（R）-2-羟基戊二酸脱氢酶及其用于将2-氧戊二酸转化为（R）-2-羟基戊二酸的反应或其反应。 本文还描述了用于产生非天然微生物生物体以将2-氧代己二酸酯酶化转化为（E）-2-己烯二酸酯或己二酸酯，或将2-氧戊二酸酯酶促转化为（E）-2-戊烯二酸酯或戊二酸酯的组合物和方法，或 各自的反向反应。